FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_A9_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_A9_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204770
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA6450.31498754700395565No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC6290.30717390242711334No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA5730.2798261464081653No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT5410.2641988572544806No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA5210.25443180153342776No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT4810.234897690091322No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4780.23343263173316403No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT4620.22561898715632175No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC4550.2222005176539532No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT4520.22073545929579527No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC4430.2163402842213215No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT4070.19875958392342627No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA3810.1860624114860575No Hit
TTCTAATGCTGTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCAC3770.18410900034184696No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT3250.15871465546710944No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC3050.14894759974605656No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT3020.14748254138789862No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA3010.146994188601846No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT3010.146994188601846No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2910.14211066074131953No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC2900.14162230795526687No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGATGAAGAA2900.14162230795526687No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT2890.14113395516921423No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA2860.13966889681105632No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC2820.13771548566684574No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC2780.13576207452263514No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA2580.12599501880158226No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT2440.11915807979684524No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2420.11818137422473995No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC2380.11622796308052936No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC2360.11525125750842409No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT2280.11134443522000292No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC2260.11036772964789764No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT2250.109879376861845No Hit
GGACAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA2170.10597255457342385No Hit
GTAATAAGTGACATGAATTTTCTTGATGAAGAAGGAAAAGCATATACAGC2160.1054842017873712No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT2150.10499584900131856No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG2100.10255408507105533No Hit
ATTCAATACATCAAGAATTGAAAAGAACCCTTCATTAAGGATGAAGTGGG2060.10060067392684476No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC2050.10011232114079212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACC100.0070867743145.04
GGTGCAC100.0070867743145.03
AGCACGC400.0145.01
CACGCAC450.0128.888893
GCACGCA450.0128.888892
AACAACG203.8616196E-4108.757
ACACGAG404.796675E-9108.752
CAACGCA203.8616196E-4108.759
GAAACAC203.8616196E-4108.754
CGGAGCC203.8616196E-4108.752
TGGTCGG203.8616196E-4108.75145
GAAGCGG352.7538408E-7103.571431
ACGCACT650.0100.384624
CGCACTT650.0100.384625
ACGTGCA301.5964084E-596.666662
GCGGTGC259.3760114E-487.01
AAACACG259.3760114E-487.05
AACACGA502.2595486E-887.01
GTAGTAA3150.082.857151
CACGTGC755.4569682E-1177.3333361
Produced by FastQC (version 0.11.5)