FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_A8_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_A8_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235582
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT7920.33618867315839074No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT5430.23049299182450272No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC4820.2045996723009398No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT4120.17488602694603153No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT3980.16894329787504986No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3870.16427401074785No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA3770.1600292042685774No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA3350.14220101705563243No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT3090.13116452020952366No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT3060.12989107826574187No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG3040.12904211696988735No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC2940.12479731049061472No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT2900.1230993878989057No Hit
AAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTATTTCAT2820.11970354271548761No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA2810.11927906206756034No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2800.11885458141963308No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT2790.1184301007717058No Hit
GTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTC2760.11715665882792405No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTTTAAAATCAATTCC2740.11630769753206952No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA2630.11163841040486966No Hit
AGTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATT2560.10866704586937881No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT2550.10824256522145156No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2540.10781808457352429No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2510.10654464262974252No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTGCTAGTTTTCCTTCT2510.10654464262974252No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2500.10612016198181524No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC2490.10569568133388799No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG2450.10399775874217895No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG2430.10314879744632442No Hit
GTATATGCTTTTCCTTCTTCGTCAAGAAAATTCATGTCACTTATTACATA2420.10272431679839715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA2950.0132.711874
GCACGCA600.0120.8333362
AGCACGC501.8189894E-12116.01
CACGCAC600.0108.753
TAACAAG3800.0101.118425
TAGTAAC4150.094.337352
GAAACAC700.093.214294
CAACGCA554.1472958E-1092.2727369
ACGTGCA554.1472958E-1092.2727362
ACAAGAG4250.092.1176457
GAAGCGG406.102873E-790.6251
ACAACAG353.4318135E-582.857141
CAGAGCA900.080.555564
AGTAACA4950.079.090913
GCAGAGC1750.074.571433
AAGCGGA300.001933899572.52
GAGCATC702.7994247E-972.56
AACACGT406.652625E-572.56
AAACACG955.456968E-1268.684215
ACAACGC754.833055E-967.6666648
Produced by FastQC (version 0.11.5)