FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_A7_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_A7_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261792
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC12890.49237562645153404No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA8880.3392005867253392No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG8060.3078780100232245No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA7390.28228517296174066No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA5930.22651570712626817No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG5500.21009045348979344No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA5280.20168683535020168No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT4880.1864075296418531No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT4840.18487959907101822No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG4300.16425253636474757No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT4160.15890477936682557No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC4130.15775883143869943No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA4120.15737684879599073No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA4120.15737684879599073No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA4050.1547029702970297No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA4050.1547029702970297No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG3970.15164710915536No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC3850.1470633174428554No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT3790.1447714215866031No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT3690.14095159515951594No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3680.14056961251680725No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC3620.13827771666055494No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA3600.13751375137513752No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC3550.13560383816159394No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT3530.13483987287617652No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG3440.13140202909179807No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA3380.1291101332355458No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA3280.12529030680845862No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT3210.12261642830949761No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3180.12147048038137147No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC3150.12032453245324534No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3120.11917858452511919No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA3020.11535875809803202No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA2930.1119209143136536No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT2920.11153893167094486No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA2910.11115694902823615No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA2910.11115694902823615No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2870.10962901845740129No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC2850.10886505317198386No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT2810.107337122601149No Hit
GGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCACCTGGGGA2770.10580919203031414No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA2680.1023713482459357No Hit
GTCCTGGAGGCTGTAAATGCTAACTTATTTGGAAAAAGGGTCTTTTCAGA2630.10046143503239213No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2620.10007945238968341No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAC100.007087893145.0145
AGCACGC3500.0132.571431
GCACGCA3550.0130.704222
ACGCACT3650.0127.123294
CGCACTT3750.0123.733345
CACGCAC4150.0111.807233
GCGGGTG203.8628338E-4108.759
AAACACG1850.097.972985
GCACTTG2450.097.653066
GCACTTC2800.095.8035666
TACCTAG259.378952E-487.07
GTAGTAA2700.083.2407461
TAGTAAC2950.076.186442
GCGGTGC1700.072.51
GTGTAAT300.001934140872.51
AACACGT1100.072.56
TAACAAG3250.069.153855
ACTTCAG3500.068.357158
ACAGCAC1700.068.235293
ACACGTG1200.066.4583367
Produced by FastQC (version 0.11.5)