FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_A4_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_A4_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252167
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG12200.4838063664159069No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA9270.3676135259570047No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC9220.3656307129798903No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA8250.3271641412238715No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG7870.3120947625978023No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA7190.28512850610904683No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT6960.27600756641432067No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC5870.23278224351322732No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT4720.18717754503959677No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC4360.1729012916043733No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA4280.1697287908409903No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG3680.1459350351156178No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA3570.14157284656596622No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG3560.14117628397054333No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG3380.13403815725293158No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC3250.12888284351243423No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGG3010.11936534122228522No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC2930.11619284045890224No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA2660.10548565038248463No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA2620.10389940000079313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1251.8189894E-1258.01
TAGAAAC3400.055.4411772
CGAAAGC1353.6379788E-1253.7037053
CAGTACG1152.4720066E-950.434789
GCGAAAG1509.094947E-1248.3333322
GAGCGAA901.3196059E-648.3333321
AGGTCGT753.8644575E-648.3333326
CAAAAGC7250.048.04
AAGGTCG803.7266625E-545.31255
GTATTAA5300.043.7735821
AAGCAGG9650.039.818657
ATTAATT5550.039.189193
GGTCGTT753.8644575E-638.6666647
GAGCAAA9400.038.563831
TTTAAAC3400.038.3823553
GTAGAAA5150.038.009711
ACTATTC3300.037.3484848
AAAGCAG10450.036.7703366
GCTATAA3550.036.7605631
TATATTC4350.035.03
Produced by FastQC (version 0.11.5)