FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_A3_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_A3_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205583
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG7260.35314203995466553No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5420.26364047610940594No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG5180.2519663590861112No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4760.23153665429534545No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4600.22375390961314898No Hit
ATCTATAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT4120.2004056755665595No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG4010.1950550385975494No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3920.1906772447138139No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3670.17851670614788187No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3670.17851670614788187No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3630.17657101997733277No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3390.16489690295403803No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3240.15760057981447884No Hit
ATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATCGG3100.1507906782175569No Hit
CTCCAATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2970.14446719816327225No Hit
TCCCTGTACACATTGTGGTCATAAGTTTCATTTCTTATTGATTCTATGCA2960.14398077662063496No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2960.14398077662063496No Hit
GTACGTGATAGGACCCCTTATCGGACTCTATTGATGAATGAGTTGGGTGT2860.1391165611942622No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2810.13668445348107577No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2720.13230665959734025No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTTCAAACAG2650.12890170879887927No Hit
GGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCGGGA2640.12841528725624202No Hit
TACTATGATTCTTCCTGATGATTGAGCATACGGGAAGATTTGGTCCTTGT2630.1279288657136047No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2630.1279288657136047No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG2570.12501033645778103No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2570.12501033645778103No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2540.1235510718298692No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2520.12257822874459463No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC2480.12063254257404551No Hit
GCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAAC2440.1186868564034964No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2420.11771401331822184No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC2360.11479548406239815No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC2340.11382264097712361No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG2340.11382264097712361No Hit
GTACACATTGTGGTCATAAGTTTCATTTCTTATTGATTCTATGCAGGCAT2280.11090411172129991No Hit
ATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC2270.11041769017866264No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG2260.10993126863602534No Hit
GAATAAGGGGTATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA2260.10993126863602534No Hit
CTATTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGTCTGGT2190.1065263178375644No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2130.10360778858174072No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT2130.10360778858174072No Hit
CTTATAGATAGTGTTGTTTCATGGTCCAAAGATATTCTCAGGACCCAGGA2110.10263494549646614No Hit
GGATACCCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGT2080.10117568086855432No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2070.10068925932591702No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2070.10068925932591702No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC100.0070867934145.03
AAACTAT352.7538772E-7103.571436
TAGAAAC1300.083.653852
AGCTTAT406.65061E-572.51
GCCTGCC451.1916114E-464.44444145
CTAAATT451.1916114E-464.444448
GCTAAAT451.1916114E-464.444447
AAGCTAA451.1916114E-464.444445
CGAAAGC808.043571E-963.43753
ACAAGGG1300.061.3461537
TATATAT606.7864403E-660.4166684
AGCAGGA753.7464088E-758.0000048
TAATGGG502.0064724E-458.04
CAAGGGT1400.056.9642878
GCAGGGT1153.8198777E-1156.7391327
AGGTGTG651.0905736E-555.769238
GGAGGTG651.0905736E-555.769236
TATGCTG1450.055.05
AAGCAGG5550.054.8648647
GTCTGCT400.00604407654.3759
Produced by FastQC (version 0.11.5)