FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_A12_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_A12_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216416
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT7810.36087904775986984No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA6190.286023214549756No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA5390.249057370989206No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4290.19822933609344967No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC4160.1922223865148603No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG4000.18482921780275025No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT3700.170967026467544No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA3630.16773251515599585No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA3620.16727044211148898No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA3550.16403593079994086No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG3460.159877273399379No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT3440.15895312731036523No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3420.15802898122135148No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT3230.14924959337572083No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG3030.14000813248558333No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA3020.13954605944107643No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT2910.13446325595150083No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT2800.1293804524619252No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT2760.12753216028389766No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT2740.12660801419488393No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2720.12568386810587018No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT2710.1252217950613633No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT2670.12337350288333579No Hit
GGAAAATACAGGGCTTAACTTCCCTTATAGACAGATGGAGCAAGAAACAT2660.12291142983882893No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC2630.12152521070530829No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2630.12152521070530829No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA2610.12060106461629454No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT2610.12060106461629454No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC2600.12013899157178767No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT2600.12013899157178767No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2580.11921484548277392No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG2510.11598033417122579No Hit
CCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACT2490.11505618808221203No Hit
CAAGTAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTT2450.11320789590418454No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT2430.11228374981517078No Hit
ATCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAA2410.11135960372615702No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT2410.11135960372615702No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG2330.10766301937010203No Hit
CTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATAGACAGATGGAG2310.10673887328108828No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA2310.10673887328108828No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG2230.10304228892503327No Hit
GTTTATATGGTCTCCAGAGACAATGTTTCTTGCTCCATCTGTCTATAAGG2220.1025802158805264No Hit
GTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCTGTATCAGTCT2210.10211814283601951No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA2200.10165606979151265No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAATTT2200.10165606979151265No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG2180.10073192370249888No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAGGAGG2180.10073192370249888No Hit
AGTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATT2170.10026985065799202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA550.0131.818192
AGCACGC550.0131.818191
CGCACTT600.0120.8333365
TAGAAAC550.0118.636372
AAACACG650.0111.538465
AACACGT203.8619197E-4108.751
GTAACAA2200.0102.1590964
CACGCAC750.096.6666643
GAAACAC700.093.214294
GAGCATC406.1011815E-790.6256
CGTGCCT259.3767385E-487.05
ACGTGCC259.3767385E-487.04
TACTATG259.3767385E-487.07
ACGCACT850.085.294114
ACGTGCA300.001933685772.53
TAGTAAC3400.070.3676452
TAACAAG3200.070.2343755
AGTAACA3600.066.4583363
GTATAAT554.0508658E-665.9090961
ACACGTG451.19175325E-464.444441
Produced by FastQC (version 0.11.5)