FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n02_A10_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n02_A10_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311353
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT6200.19913089001872475No Hit
CAACCAAGACTACTCGTTAAGTAATGAATCCTCATTGGATGAGGAAGGGA5880.18885316666291957No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT5750.18467784154962374No Hit
CATGTTAACTTTTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAG5260.1689400776610471No Hit
GGAATTTCCATTGCAAAATTAGATACAAATCCATCTCTATAGAACATGCT4830.15512938690168393No Hit
ACTCATTTCCTCATTACTTATTGTATTTACACAGTCTCTCCAAAGCTTCC4750.15255995606273265No Hit
CATTTCAACTTCTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAG4700.1509540617883881No Hit
GGAATTTCCATTGCAAAATTAGATACAAATCCATCTCTGTAGAACATGCT4640.14902698865917463No Hit
ATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA4520.1451728424007477No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT4470.14356694812640317No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT4460.14324576927153423No Hit
GTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTCCAGAATACATT4230.1358586556095493No Hit
GTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTGATTGA4210.13521629789981146No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT4150.133289224770598No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC4010.12879272080243326No Hit
GGGTCTATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACC3890.12493857454400632No Hit
GTTATTCATAGCTGATTATAGATACACCTACAAATGCCACAGGGGAGATT3820.12269032255992394No Hit
TCTCTGGACCATCCATGCTATGGTTCTTGGCATTCCCTCAATTACTTCAT3780.1214056071404483No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAACTT3740.12012089172097266No Hit
GGGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACC3530.11337613576872553No Hit
CAAAAGGATTGGCCGATGATTACTTTTGGAAAAAGAAAGAAAAGCTGGGA3450.11080670492977425No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC3440.11048552607490533No Hit
GGTTTATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCA3350.10759491638108512No Hit
GACTCAGGAAAGTGGCCAAAATATACTGTGTTTAGGATTGGCTCCCTATT3350.10759491638108512No Hit
GTCCAAAGGTCCTTAGCTCAAGAGCATGGAATAGAGACTCCTAAGTATCT3270.10502548554213384No Hit
ATTGCGTTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACC3170.10181369699344474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA650.0122.6923142
AGCACGC650.0122.6923141
CAACGCA256.457829E-6116.09
GTGTAGG203.8635297E-4108.75145
CACGCAC750.0106.3333363
ACGCACT900.088.6111154
CGCACTT900.088.6111155
CGATTGG502.2635504E-887.01
ACGTGCA502.2635504E-887.02
AAACACG1000.087.05
ACAACGC353.4335517E-582.857148
GAAACAC1050.082.857144
CTAACCA755.638867E-1177.3333364
TAGAAAC1200.072.52
TACTGGA502.302506E-672.52
CGGTGCG300.001934486372.52
AACACGT406.6559885E-572.56
TCTAAGG1750.066.285712
ACTGGAC554.055042E-665.9090963
TAGTAAC3250.062.4615362
Produced by FastQC (version 0.11.5)