FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286866
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19870.6926578960211388No Hit
GCATTAGCAGATGCTTTATGGTATGTGTCATGAATACAAATGACAGCCAA10140.353475141703792No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC3950.13769495164989926No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT651.6370905E-1189.230775
CCTAGGT850.085.294124
TAGGTAG950.076.315796
ACCTAGT350.003563911762.1428578
TCCTAGG1251.8189894E-1258.03
GTGCCCG502.0078175E-458.09
GCATTAG3300.057.1212121
CTAGGTA1353.6379788E-1253.7037055
GCACGCA1004.6875357E-850.752
TTAGCAG3900.048.3333364
ACGCACT1255.175025E-946.3999984
TAGCAGA4000.045.31255
GCAGGTA1453.8198777E-1045.09
AGGTAGT1703.8198777E-1142.647067
GAGGTCG700.001055380241.428579
CGAGGTC750.00148162138.6666688
CAGGTCA800.00203419936.258
GTTCCTA2201.6370905E-1136.251
CACGCAC1655.9912054E-835.1515163
TAACAAG2302.5465852E-1134.6739165
Produced by FastQC (version 0.11.5)