FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E9_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E9_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117963
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA3290.2789010113340624No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2910.2466875206632588No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT2640.22379898781821417No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG2620.22210354094080348No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC2590.2195603706246874No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2560.21701720030857133No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2400.20345362528928565No Hit
GATCTGGACGTAGCCTTGGGCAGACCAAAATGCACGGGGAAAATACCCTC2350.19921500809575884No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC2030.17208785805718743No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA2010.17039241117977671No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT1960.16615379398624994No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA1950.16530607054754456No Hit
GCTCTATGGGGACTCAAAGCCCCAGAAGTTCACCTCATCTGCCAACGGAG1920.1627629002314285No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1840.15598111272178564No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT1760.1491993252121428No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT1740.14750387833473208No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG1680.14241753770249993No Hit
GTTTAGAACAGACTCAACCATTCCTCCATTACAGAGCCATGTCAACACCT1640.1390266439476785No Hit
GCATTATTGTCAGGGCCATCAACTCCGATATATGTCCATTCCTTACCATC1610.13648347363156244No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA1600.1356357501928571No Hit
CCATCATACTTGACATACAGTCCCATCCCCATCCTTTCAGTTTTAGACAT1600.1356357501928571No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC1580.13394030331544637No Hit
CCATTGGATTGTGCAAACGCATCAAATGTTCAGGCTGTGAACCGTTCTGC1540.13054940956062494No Hit
GCTTGTTTCTGTCTCCTCTTGTTCCATTGTAGTATCCCCCTGGTTGGGCT1530.12970168612191957No Hit
CCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACT1530.12970168612191957No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTACTTTGAGCAATG1520.12885396268321422No Hit
CTCCTAATTAGCCCTCATAGATTCGGAGAAACCAAAGGAAACTCAGCTCC1440.12207217517357137No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA1440.12207217517357137No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG1420.12037672829616065No Hit
GTCCAGATCTGTGCAGTTGAGGCATTTTGGGCATAGTTTCCCCCTGGTTT1410.11952900485745531No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT1410.11952900485745531No Hit
CTCTTAAGGTCTGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTA1410.11952900485745531No Hit
GTCCCATCCCCATCCTTTCAGTTTTAGACATCGTTCGAGAGTACCACCTT1390.11783355798004459No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT1360.11529038766392853No Hit
GGGATAACATCGTCAAACTCACCACATGTCGTCAAAACTGCTACTCAAGG1360.11529038766392853No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA1350.11444266422522317No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA1350.11444266422522317No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGTTTCCATTGGTAATGTTAGGGCA1340.1135949407865178No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT1340.1135949407865178No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG1330.11274721734781244No Hit
GACACATACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGC1300.11020404703169638No Hit
GTCCTGAGCCCATTAAACAGTAAATGGCTGTTGCTGCTGAGTGCCAAGTC1270.1076608767155803No Hit
ATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAGGAGTA1240.10511770639946424No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA1240.10511770639946424No Hit
CAGTTACACAGCAAAAAGACCTTTTGTCAAATTAAACGTGGAGACTGATA1220.10342225952205351No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT1220.10342225952205351No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT1200.10172681264464283No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1200.10172681264464283No Hit
AGTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATT1180.1000313657672321No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAG100.007082991145.05
GAAACAC306.4574124E-10145.04
AAACACG306.4574124E-10145.05
ACGAGCC151.2272777E-4145.02
CGAGCCT151.2272777E-4145.03
GTAACAA1200.0132.916674
TAACAAG1400.0113.928575
CACGCAC203.857515E-4108.753
GAAGCGG203.857515E-4108.751
CACTGCC203.857515E-4108.757
GCACGCA203.857515E-4108.752
AGCACGC203.857515E-4108.751
GAGCATC553.6379788E-12105.4545446
ACACGAG352.7465467E-7103.571437
AACACGA352.7465467E-7103.571436
TAGAAAC451.0832082E-896.6666642
ACGTGCA353.424696E-582.857142
TAGTAAC2050.077.804882
AGCATCT755.4569682E-1177.333337
CCGTGTT300.001931497172.5145
Produced by FastQC (version 0.11.5)