FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E8_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E8_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222224
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT8420.3788969688242494No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG3850.1732486140110879No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC3430.15434876520987834No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC3270.14714882280941752No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA3190.14354885160918712No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT2960.13319893440852473No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA2750.12374901000791995No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA2730.12284901720786233No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2700.12149902800777594No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA2590.11654906760745914No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT2570.11564907480740153No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT2520.11339909280725755No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG2480.11159910720714235No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC2410.10844913240694075No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2390.10754913960688314No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT2380.10709914320685433No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG2360.10619915040679674No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC2350.10574915400676795No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT2290.10304917560659516No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCCG100.0070871776145.01
CGGTGCG100.0070871776145.02
TACTAGA151.228728E-4145.01
CATCCGC100.0070871776145.02
GTGCGTT100.0070871776145.04
CACGCAC1250.0133.43
GCACGCA1250.0133.42
AGCACGC1250.0133.41
AAACACG900.0120.8333365
ACAACGC256.453727E-6116.08
GCGGTGC256.453727E-6116.01
GAAACAC950.0114.473694
ACGCACT1500.0111.166674
CGCACTT1500.0111.166675
GAGTAGG203.8620573E-4108.75145
TAGAAAC1150.0100.869572
CAACGCA301.596709E-596.6666649
ACGTGCA451.0877557E-896.6666642
AACACGA900.088.6111156
AGCGTTT259.377074E-487.06
Produced by FastQC (version 0.11.5)