FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E7_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E7_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218675
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC10260.4691894363781868No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA7740.3539499256888076No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA6550.2995312678632674No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG5630.2574597004687321No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA5590.25563050188636105No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG5550.2538013033039899No Hit
CACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA5180.23688121641705726No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT5100.23322281925231506No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA5050.2309363210243512No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA4950.22636332456842345No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG4690.21447353378301134No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT4630.21172973590945468No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA4280.19572424831370755No Hit
GTAGTAACAAGAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTA4230.19343775008574368No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA4140.1893220532754087No Hit
CATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG4110.18795015433863038No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG3650.16691437064136275No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT3590.1641705727678061No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3450.15776837772950725No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT3330.15228078198239395No Hit
CACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA3230.1477077855264662No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3190.1458785869440951No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT3140.14359208871613124No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC2970.13581799474105408No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2880.1317022979307191No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC2880.1317022979307191No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA2870.13124499828512634No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG2800.1280439007659769No Hit
ACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACC2800.1280439007659769No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2780.12712930147479135No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC2780.12712930147479135No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA2700.12347090431004916No Hit
GTAGTAACAAGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT2690.12301360466445638No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA2650.12118440608208528No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT2630.12026980679089973No Hit
GCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCAT2620.11981250714530696No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC2620.11981250714530696No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT2610.11935520749971418No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA2590.11844060820852863No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA2570.11752600891734308No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT2540.11615410998056476No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2480.11341031210700811No Hit
GGCATGATGTGTCCCCCATGGCCATTGAAGAGTTGATTTGAACGATGACG2480.11341031210700811No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC2470.11295301246141536No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT2440.11158111352463702No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC2400.10975191494226592No Hit
CACGTGCATATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGC2370.1083800160054876No Hit
ACCCAATGGTGTTCTCGCAACTCTACGGTATCCTGCAGCAGGAAACGAAT2360.10792271635989482No Hit
GTCCCATGGTGGGTGAAAATCTATCCTGGATAACTCCTGGACCAGTACCA2350.10746541671430206No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT2330.1065508174231165No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAA151.2287016E-4145.05
AGCACGC2500.0133.41
TAGTAAC2100.0127.738092
GCACGCA2750.0121.272732
CGCACTT2900.0117.55
AGTAACA2550.0110.882353
ACGCACT3100.0109.919354
CACGCAC3500.095.285713
GAAGCGG1100.092.2727361
GCACTTG2050.091.951226
TAACAAG3050.090.327875
GTAGTAA3000.089.416671
ACGTGCG259.376871E-487.02
GTGCGCT259.376871E-487.04
AAACACG1350.085.925925
GCACTTC2200.082.386376
CGCTCGT451.229655E-680.555567
GTAACAA3650.075.479454
GCGGTGC1350.075.185181
ACGTGCA1400.072.52
Produced by FastQC (version 0.11.5)