FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E6_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E6_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162427
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4560.28074150233643425No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT3630.22348501172834564No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC3420.21055612675232568No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC3350.20624649842698567No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3250.2000898865336428No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT3040.1871610015576228No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT2820.17361645539226853No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA2650.16315021517358566No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC2250.13852376760021426No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC2190.13482980046420853No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT2080.1280575273815314No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA2080.1280575273815314No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC2060.12682620500286282No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC1900.11697562597351426No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT1890.11635996478417997No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC1890.11635996478417997No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT1880.11574430359484568No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA1820.11205033645883998No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG1820.11205033645883998No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT1790.11020335289083712No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC1790.11020335289083712No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT1780.10958769170150283No Hit
CCCATACACCGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA1770.10897203051216856No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG1740.10712504694416569No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA1700.10466240218682855No Hit
CTCCTAGACTCGGCACTAAACCCCTCCAATTGGGCATTTCCAAATACATA1690.10404674099749427No Hit
CCCTACATGCTCCACAGGTGCTACGTCCGGCCCTATTTCTGGTAGGTCCA1690.10404674099749427No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT1650.10158409624015713No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCC100.0070854323145.00002145
CACGCAC550.0145.000023
GCACGCA550.0145.000022
CAACGCA100.0070854323145.000029
AGCACGC600.0132.916671
GAAACAC351.891749E-9124.285714
AAACACG351.891749E-9124.285715
ACGCACT650.0122.6923144
CGCACTT650.0122.6923145
ACGTGCA203.860164E-4108.750012
ATATAGG203.860164E-4108.750013
TAGAAAC502.2566383E-887.02
GCGTTTT259.3724934E-487.07
AACACGT259.3724934E-487.06
GCACTTT1400.072.56
AGCGTTT300.001932813872.56
GTAGTAA2000.072.51
AACACGA300.001932813872.56
ACACGTG350.00356112862.1428577
ACACGAG350.00356112862.1428577
Produced by FastQC (version 0.11.5)