FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E5_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E5_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170391
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4480.26292468498923066No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT3850.22595090116262012No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT3720.21832139021427188No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT3000.1760656372695741No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2840.16667546994853014No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2800.16432792811826916No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2790.1637410426607039No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2580.15141644805183374No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2550.149655791679138No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC2530.1484820207640075No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC2510.147308249848877No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA2460.14437382256105077No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC2390.14026562435809403No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2390.14026562435809403No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC2140.12559348791896285No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT2100.12324594608870186No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2000.1173770915130494No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT1930.11326889331009268No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG1920.11268200785252741No Hit
GATCTATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGC1880.11033446602226643No Hit
CCCCATAAACATCTTCGAAGCTTATATGTACCCAATCTCACCAAGGTGAG1860.10916069510713594No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT1850.1085738096495707No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC1840.10798692419200545No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT1810.1062262678193097No Hit
ACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAACAAGGGGAAA1770.10387872598904872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACAG202.1254818E-6145.01
TTATCTA100.0070857364145.04
GAAACAC700.0124.285714
AAACACG700.0124.285715
GTAACAA1850.0109.729744
AAGCACG203.8604933E-4108.751
CACGCAC650.0100.384613
GCACGCA650.0100.384612
AGCACGC650.0100.384611
TAGAAAC1000.094.252
ACGTGCA406.095579E-790.6252
ACGCACT750.087.04
CGCACTT750.087.05
AACACGA801.8189894E-1281.56256
TAGTAAC2600.078.076922
TAACAAG2750.073.8181845
CAGAAGC502.298024E-672.51
ACACGAG903.6379788E-1272.57
AAGCGGA300.001932977172.51
GTATGAG406.647343E-572.52
Produced by FastQC (version 0.11.5)