FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E4_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E4_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176908
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG11400.6444027404074434No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC8140.460126167273385No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG6360.3595088972799421No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA6140.34707305492120194No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA5690.3216361046419608No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA4570.2583263617247383No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA4480.25323897166889003No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA4140.23401994256901892No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC4100.23175888032197525No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA3860.2181925068397133No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC3830.21649671015443053No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT3510.19840821217808127No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC3480.19671241549279853No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA3360.18992922875166754No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA3270.1848418386958193No Hit
GGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCACCTGGGGA3260.1842765731340584No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC3030.17127546521355733No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2940.1661880751577091No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT2940.1661880751577091No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2920.16505754403418726No Hit
AAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA2790.15770909173129535No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA2750.15544802948425168No Hit
GATATAGCCACAGCCAAGGCATGCTGGCGGCCGCCAGAAGCTGGAAGAGG2650.14979537386664254No Hit
GTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGACGTGAAAGCTGAAGA2630.14866484274312072No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA2600.14696904605783798No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT2560.1447079838107943No Hit
GAAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG2480.140185859316707No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG2460.13905532819318517No Hit
GTAGTAGGTTGAATAATTCCCTGCCCTCTCCCACTCAGTCCTCAAAGATA2410.13622900038438057No Hit
GTCCTGGAGGCTGTAAATGCTAACTTATTTGGAAAAAGGGTCTTTTCAGA2410.13622900038438057No Hit
CCTTATAAGAGACAAGCAGAGGGAGATGTAACATGCAGGCACACAACAGG2360.133402672575576No Hit
CTCTCTGCCCAGTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCT2330.13170687589029326No Hit
TTTCAGATGTGATTAAGTATCTTGAGATGGGGAGAGTATTCTGGATTATT2260.12775001695796684No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2120.11983629909331403No Hit
CCCTCTCTACCTTGCATGGTCTCTGCCCAGGACGCACCTCTGCCTAAGGG2120.11983629909331403No Hit
ATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCTTGCTT2100.11870576796979221No Hit
GTCCTACTGCAGCCTGTTGCCAAGGAGCTCCCCACAGACATGGAGCTCTC2080.11757523684627039No Hit
CACTAAAGGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCAC2070.11700997128450946No Hit
GCATAACTCAGTGTTACAAGCTGGGCTTTAAAGCCAGGTTCTCTGGTTTA2040.11531417459922672No Hit
CCCTACAGCCACTTCTTCAGAAATTGAACTTGCTCTAAAATCCACTTCAT2030.1147489090374658No Hit
GTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGCTCACTCTCAC1980.11192258122866122No Hit
GCCTCTTACAGTGCATTAGTTTCTGTAGCTGCTGTAACAGGGTACCACAA1950.11022678454337848No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT1930.10909625341985665No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC1910.10796572229633482No Hit
GGGCAGAGAGTGTACCTCAAAACAAGGTGTACTTAAAAGAGAATCTGGGA1890.10683519117281298No Hit
GCCGTATAGTGGATGCTGTCATCGAACAAGTCAAGGCCGCTGGAGCACTG1870.10570466004929116No Hit
GTTCTGGAGGCCAGAAATTTGAAATCAGCATCACTAGGCTGAAATCAAGG1860.10513939448753024No Hit
GTGTAGCAGCTATGCTCCCTACAGCCACTTCTTCAGAAATTGAACTTGCT1850.10457412892576932No Hit
CTCCCACTCAGTCCTCAAAGATAGCAGGCCCTAGTCCTGGAGGCTGTAAA1830.10344359780224749No Hit
CCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGTGACAGGC1820.10287833224048658No Hit
GTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCTTGGAAGCCAGA1820.10287833224048658No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT1800.10174780111696476No Hit
GTATAGTGGATGCTGTCATCGAACAAGTCAAGGCCGCTGGAGCACTGCTT1770.10005200443168201No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCG202.125711E-6145.09
CGGTGCG100.0070859645145.02
AGCACGC3000.0123.249991
GCACGCA3100.0119.274192
ACGCACT3150.0117.380964
CACGCAC3150.0117.380963
GCACTTG1950.0107.820526
CGCACTT3550.0104.154935
TAGTAAC1750.099.4285662
GCACTTC2000.097.8756
TAGAGTA301.5958043E-596.6666645
CCCCGTT301.5958043E-596.6666649
GTATGCT301.5958043E-596.6666649
CACTTGA2150.091.046517
GCGGTGC1400.088.035711
ACTTCAG2000.087.08
CGGTGCC259.37389E-487.02
ACACGTG353.4294448E-582.857147
CCCCCGT353.4294448E-582.857148
AAACACG1000.079.755
Produced by FastQC (version 0.11.5)