FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E3_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E3_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218008
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG22241.02014604968625No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC20240.9284062970166232No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA20210.9270302007265788No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC19200.8807016256284174No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA18900.8669406627279732No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA17080.7834574877986129No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG16250.7453854904407178No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT14030.643554364977432No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGG10330.47383582253862244No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT9370.4298007412572016No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC9260.42475505486037207No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA8760.4018201166929654No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA7610.34906975890792996No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC6970.3197130380536494No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA6730.3087042677332942No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG6460.29631940112289457No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG6310.28943891967267255No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG5780.26512788521522146No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACG5740.2632930901618289No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC5670.260082198818392No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG5660.25962350005504387No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC5600.25687130747495507No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT5460.25044952478808113No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA5240.24035815199442223No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA5180.2376059594143334No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG5130.23531246559759275No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT4880.22384499651388942No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA4550.20870793732340095No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA4450.20412094968991962No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4430.20320355216322336No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC4280.19632307071300137No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4250.19494697442295697No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT4200.1926534806062163No Hit
GTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGTTTGTCCCTAAGAG4100.18806649297273492No Hit
CCTCAATTGCTTCGTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGT4080.18714909544603867No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG4060.1862316979193424No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA4060.1862316979193424No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTC4030.184855601629298No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC3980.18256210781255736No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACG3900.17889251770577227No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3790.1738468313089428No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATG3780.17338813254559465No Hit
CTCCTCAATTGCTTCGTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA3740.1715533374922021No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC3600.16513155480532823No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC3550.16283806098858758No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC3550.16283806098858758No Hit
TCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATT3460.15870977211845438No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3360.15412278448497305No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT3200.14678360427140288No Hit
ATTCAATAGCTTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT3160.14494880921801034No Hit
CATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGATGCTTAAT3110.1426553154012697No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG3080.14127921911122526No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT3040.13944442405783272No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2990.13715093024109207No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT2950.13531613518769953No Hit
CTCCAATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2940.1348574364243514No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2920.13394003889765513No Hit
GAGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC2880.13210524384426262No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCGTATAGCCCCCCAAGATCAAA2730.12522476239404057No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2690.12338996734064804No Hit
GTATTCAATAGCTTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA2680.12293126857729991No Hit
GAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT2660.12201387105060366No Hit
ATGCTAAGTCAGTATTCAATAGCTTGTATGCATCACCACAATTGGAAGGA2630.12063777476055924No Hit
CTTAGGGACAAACTCGAACCTGGGACCTTTGATCTTGGGGGGCTATACGA2560.1174268834171223No Hit
GCTTCAACCCGATGATTGTCGAACTTGCAGAAAAAGCAATGAAAGAGTAT2560.1174268834171223No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCGTATAGCCCCCC2530.1160507871270779No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCACT2520.11559208836372976No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG2520.11559208836372976No Hit
ATCTATAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT2490.11421599207368538No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2400.11008770320355217No Hit
GGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGA2400.11008770320355217No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2390.10962900444020401No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATC2350.1077942093868115No Hit
GGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCAT2350.1077942093868115No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2330.10687681186011522No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2290.10504201680672269No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT2270.10412461928002642No Hit
GCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACT2250.10320722175333015No Hit
AAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTT2230.1022898242266339No Hit
GAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT2210.10137242669993761No Hit
GTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTT2210.10137242669993761No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA2200.10091372793658948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTG100.007087087145.05
CACTAAG100.007087087145.02
GGACCGG100.007087087145.01
GCAGGGT458.367351E-11112.777789
GAGCGAA2150.091.046511
CAAAAGC9400.071.728724
AAGCAGG12500.069.67
TAGAAAC3100.067.822582
CGAAAGC2950.066.3559344
AGTAAAG554.0509676E-665.909092
GCGAAAG3100.063.1451643
AGCAGGT10950.062.2374428
AAACTAT3750.059.933336
GCAGGTC5700.059.7807049
GCAGGTA5700.059.7807049
AAAGCAG14950.058.193986
AGCGAAA3400.057.5735322
GTAGAAA3700.056.824321
CAAGGTA1550.056.1290329
TTTAAAC4050.055.4938283
Produced by FastQC (version 0.11.5)