FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E1_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E1_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193901
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC301.5961936E-596.6666643
ACGCTTA300.001933381872.56
AGTAACG300.001933381872.52
CGCTTAC300.001933381872.57
TAACGCT350.003562170562.1428574
TATAGAG553.2125242E-452.7272722
TAGAAAC2050.049.5121962
ATAGTGG752.5429179E-548.3333326
GCTTACC803.723455E-545.31258
CTTATAG1002.722698E-643.53
TTATAGT1803.1723175E-936.254
GTAGAAA3250.035.6923071
GAGCAAA4550.035.0549431
AACTATC900.003621077632.222227
CAAAAGC5050.030.1485144
GGATAGG1504.3806715E-529.01
TATAGTG1504.3806715E-529.05
TACACAG1255.1387196E-429.05
AAGCAGG6400.028.3203127
TCTTAGG1555.477916E-528.0645142
Produced by FastQC (version 0.11.5)