FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E12_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E12_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184698
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT6880.372499972928781No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT4370.2366024537352868No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA3360.18191859143033492No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3320.17975289391330712No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC3220.17433865012073763No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA3070.1662172844318834No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC2940.15917876750154306No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT2840.15376452370897356No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC2710.14672600677863323No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA2700.1461845823993763No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT2580.13968748984829288No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC2490.13481467043498035No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT2470.13373182167646644No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT2470.13373182167646644No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT2410.13048327540092475No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2410.13048327540092475No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC2410.13048327540092475No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC2370.12831757788389694No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC2350.12723472912538306No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT2290.12398618284984136No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA2270.12290333409132746No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC2240.12127906095355662No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT2230.12073763657429967No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTTTGG2200.11911336343652881No Hit
CCCATACACCGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA2150.11640624154024408No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA2150.11640624154024408No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA2140.11586481716098712No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2130.11532339278173018No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGA2100.11369911964395933No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT2050.11099199774767458No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC2030.10990914898916068No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT2010.10882630023064678No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG2000.10828487585138984No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCGGGCACTGAAG1990.10774345147213289No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTTGATAAAG1970.10666060271361899No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT1950.10557775395510509No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT1940.10503632957584814No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT1940.10503632957584814No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT1930.10449490519659119No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC1920.10395348081733424No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG1900.10287063205882034No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC1900.10287063205882034No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT1870.1012463589210495No Hit
CACGTGCATTTTTCACTCAATTGTATTCATTGAATTAATGGATAAATTTA1860.10070493454179255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTGTG151.2283942E-4145.07
CGGTGCA100.007086214145.02
GAGCAGA256.450815E-6116.01
AACACGT458.367351E-11112.777796
GAAGCGG553.6379788E-12105.4545441
CACGCAC1100.0105.4545443
GCACGCA1100.0105.4545442
AGCACGC1100.0105.4545441
ACAACGC352.7527676E-7103.571438
CAACGCA352.7527676E-7103.571439
GAAACAC1050.0103.571434
AAACACG1050.0103.571435
ACACGTG608.240022E-1084.5833367
TAGAAAC1300.083.653852
ACGCACT1400.082.857144
CGCACTT1400.082.857145
CGTTTGA451.2288947E-680.555562
AACACGA755.4569682E-1177.333336
GTAGTAA2600.075.288471
AACAACG502.2987897E-672.57
Produced by FastQC (version 0.11.5)