FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E11_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E11_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191456
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT10870.56775447100117No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG6520.34054821995654355No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC6040.3154771853585158No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG5270.27525906735751293No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA4620.24130870800601706No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA4410.2303401303693799No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC3810.19900133712184523No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3610.18855507270600033No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC3580.1869881330436236No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA3490.1822873140564934No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3000.1566939662376734No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA2860.14938158114658198No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT2770.14468076215945178No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG2720.14206919605549057No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC2620.13684606384756812No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2550.1331898713020224No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT2470.12901136553568443No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT2340.12222129366538527No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA2290.11960972756142403No Hit
AGCACAGCCCCCGTTCCCTCCACGTTGCCATCAGCATCCTCGGCCCTGGC2290.11960972756142403No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2250.11752047467825505No Hit
GGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCACCTGGGGA2190.1143865953535016No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC2120.11073040280795587No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC2100.10968577636637138No Hit
CTCTCTGCCCAGTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCT2100.10968577636637138No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA2090.10916346314557915No Hit
GTCTAAAGGATACCCAAATGAACGTTTTGAACTTCTCACCAACTTTCCTC2060.10759652348320242No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT2040.10655189704161791No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT1930.10080645161290322No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGC100.0070864162145.08
GCGAGTA100.0070864162145.09
GCACGCA4100.0129.085362
AGCACGC4150.0127.530121
CACGCAC4450.0118.932593
ACGCACT4600.0115.054344
CGCACTT4600.0115.054345
GCACTTC1900.0110.65796
GCGGAGT607.2759576E-1296.6666646
CCCCCGT700.093.214298
CAACGCA554.129106E-1092.272729
ACACGTG406.098471E-790.6257
TAGTAAC1750.087.02
ACAACGC608.240022E-1084.5833368
TAACAAG1850.082.2972955
GCCCCCG801.8189894E-1281.56257
CCCCGTT801.8189894E-1281.56259
CACTTGA1550.079.516137
GAAGCGG1150.075.652171
AGTAACA2050.074.2682953
Produced by FastQC (version 0.11.5)