FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_E10_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_E10_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111812
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT3600.3219690194254642No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT2490.22269523843594605No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2210.19765320359174327No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1760.15740707616356026No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1720.15382964261438845No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA1630.14578041712875184No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC1620.14488605874145888No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC1600.14309734196687296No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA1520.1359424748685293No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG1510.13504811648123635No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA1500.1341537580939434No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA1460.13057632454477158No Hit
GATCTGGACGTAGCCTTGGGCAGACCAAAATGCACGGGGAAAATACCCTC1400.12521017422101385No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG1360.12163274067184202No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC1280.11447787357349837No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA1210.10821736486244769No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1210.10821736486244769No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA1190.10642864808786177No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA1180.10553428970056881No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT1170.10463993131327586No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCATC100.007082501145.07
GTAACAA1100.0131.818194
GCACGCA403.274181E-11126.8752
AGCACGC403.274181E-11126.8751
ACACGAG256.43959E-6116.02
AACACGA256.43959E-6116.01
CACGCAC458.367351E-11112.777783
ACGCACT501.9281288E-10101.54
CGCACTT501.9281288E-10101.55
TAGTAAC1400.098.392862
CACGAGC301.5932223E-596.6666643
TCCTAGT259.364797E-487.06
CAGAGCA259.364797E-487.05
ACGAGCA259.364797E-487.04
CGAGCAT259.364797E-487.05
TAACAAG1750.082.857155
TAAATCC651.54796E-978.076924
ACAAGAG1900.076.315787
AGTAACA1850.074.4594653
ATCCTAG300.001931232572.55
Produced by FastQC (version 0.11.5)