FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_D9_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_D9_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186929
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT4670.2498274746026566No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4560.24394288740644843No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT4130.22093950109399826No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC3770.20168085208822603No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3600.19258649005772246No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC3310.17707257835862814No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA3310.17707257835862814No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT3240.1733278410519502No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT3180.17011806621765482No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT2510.13427558056802313No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2490.13320565562325803No Hit
GTCCAAAGGTCCTTAGCTCAAGAGCATGGAATAGAGACTCCTAAGTATCT2480.13267069315087546No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2440.13053084326134523No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA2380.12732106842704985No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC2340.1251812185375196No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA2340.1251812185375196No Hit
GAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAG2260.1209015187584591No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA2260.1209015187584591No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC2250.12036655628607654No Hit
CTCCTAGACTCGGCACTAAACCCCTCCAATTGGGCATTTCCAAATACATA2220.11876166886892883No Hit
CCCATACACCGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA2130.11394700661748577No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT2100.1123421192003381No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG2090.11180715672795553No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGATGAAGAA2080.11127219425557297No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC2070.11073723178319042No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA2020.10806241942127759No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC2020.10806241942127759No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC1950.10431768211459966No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC1920.10271279469745197No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT1900.10164286975268685No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA1880.10057294480792173No Hit
CCCCATAGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG1880.10057294480792173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC306.475602E-10145.01
CACGCAC351.893568E-9124.285713
GCACGCA351.893568E-9124.285712
ACGTGCA352.752895E-7103.571432
CACGTGC651.4551915E-1189.230771
CGTGCCT259.374724E-487.03
ACGTGCC259.374724E-487.02
GAAACAC353.429954E-582.857144
ACGAGCC451.2289529E-680.555564
TAGAAAC502.2989007E-672.52
AAACACG406.649031E-572.55
ACGCACT607.9959136E-872.54
GAAGCGG406.649031E-572.51
CGAGCCT406.649031E-572.55
CGCACTT607.9959136E-872.55
AACACGA651.3918361E-766.923071
ACAGCAG554.0487066E-665.909095
TTAGAGC451.1913295E-464.444443
ACACGAG702.3241773E-762.1428572
GCGTTTG702.3241773E-762.1428579
Produced by FastQC (version 0.11.5)