FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_D8_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_D8_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199133
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4510.2264817985969176No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC3150.15818573516192697No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT3130.15718138128788298No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA3130.15718138128788298No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT3020.15165743498064108No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2840.14261825011424525No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT2650.13307688831082745No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2600.1305660036257175No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2560.1285572958776295No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC2550.12805511894060753No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2530.12705076506656354No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG2350.11801158020016773No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC2210.11098110308185986No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC2210.11098110308185986No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2170.1089723953337719No Hit
GTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTC2130.10696368758568395No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT2120.10646151064866195No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA2120.10646151064866195No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2070.103950625963552No Hit
AGTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATT2040.10244409515248604No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2010.10093756434142005No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG100.0070866286145.02
AGAAGCG151.2285379E-4145.02
ATAATTA100.0070866286145.02
AGGGACG100.0070866286145.07
TAATTAC100.0070866286145.03
GGGACGT100.0070866286145.08
GGAGCGT100.0070866286145.03
GGACGTG100.0070866286145.09
CAACGCA151.2285379E-4145.09
CATAAGG151.2285379E-4145.01
GTAACAA1750.0120.142864
ATCTTGA203.8614613E-4108.75145
CACGCAC352.753568E-7103.571433
AGCACGC352.753568E-7103.571431
GAGCATC301.5962994E-596.6666646
GCACGCA406.0993807E-790.6252
GAAACAC900.088.6111154
GAGCGTT259.375631E-487.04
AGCGTTT259.375631E-487.05
TAACAAG2500.084.1000065
Produced by FastQC (version 0.11.5)