FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_D7_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_D7_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181354
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG9020.4973697850612614No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC7480.4124529924898266No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG5840.3220221224786881No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA4700.2591616396660674No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA4680.25805882417812676No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA4190.2310398447235793No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA4030.22221732082005358No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3790.20898353496476507No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC3720.20512368075697257No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC3640.20071241880520974No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT3520.19409552587756543No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG3510.19354411813359507No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC3370.18582440971801006No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA3210.17700188581448437No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3170.17479625483860295No Hit
GGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCACCTGGGGA3170.17479625483860295No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA3160.1742448470946326No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA3120.17203921611875117No Hit
GTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGACGTGAAAGCTGAAGA3050.16817936191095867No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT3010.16597373093507725No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT2900.15990824575140333No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC2850.15715120703155155No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA2700.14888009087199622No Hit
CTCTCTGCCCAGTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCT2700.14888009087199622No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC2600.14336601343229266No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG2530.13950615922450016No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT2520.1389547514805298No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT2480.13674912050464838No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA2480.13674912050464838No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT2300.12682378111318193No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2250.12406674239333017No Hit
GTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCTTGGAAGCCAGA2220.1224125191614191No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2200.12130970367347839No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA2200.12130970367347839No Hit
CACTAAAGGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCAC2180.12020688818553768No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT2180.12020688818553768No Hit
GTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGCTCACTCTCAC2170.11965548044156732No Hit
GCCGTATAGTGGATGCTGTCATCGAACAAGTCAAGGCCGCTGGAGCACTG2160.11910407269759696No Hit
ATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCTTGCTT2150.1185526649536266No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA2140.11800125720965625No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC2120.11689844172171554No Hit
GAAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG2120.11689844172171554No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2110.11634703397774518No Hit
GTCCCATGGTGGGTGAAAATCTATCCTGGATAACTCCTGGACCAGTACCA2110.11634703397774518No Hit
GGCATGATGTGTCCCCCATGGCCATTGAAGAGTTGATTTGAACGATGACG2100.11579562623377482No Hit
GTCCTACTGCAGCCTGTTGCCAAGGAGCTCCCCACAGACATGGAGCTCTC2060.1135899952578934No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG2060.1135899952578934No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC2060.1135899952578934No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT2040.11248717976995268No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2030.11193577202598234No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2010.11083295653804162No Hit
GATATAGCCACAGCCAAGGCATGCTGGCGGCCGCCAGAAGCTGGAAGAGG1950.10752451007421948No Hit
GTCCAGCCCTGAGTTGGGTCCTCTCACTAAAGGACTAGAGAGTGCTTTTA1940.10697310233024912No Hit
AAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA1920.10587028684230843No Hit
CTCTTGACCTGTTTGGCTTTAAAATCTGGGAGAGAGACACATCACTGCCA1910.10531887909833806No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT1890.10421606361039734No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT1890.10421606361039734No Hit
GGGCAGAGAGTGTACCTCAAAACAAGGTGTACTTAAAAGAGAATCTGGGA1890.10421606361039734No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA1870.10311324812245665No Hit
CCCTCTCTACCTTGCATGGTCTCTGCCCAGGACGCACCTCTGCCTAAGGG1870.10311324812245665No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA1860.10256184037848629No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAGCG100.0070861103145.09
TCGTGGC100.0070861103145.03
CGCTCGT100.0070861103145.07
TAGTAAC2250.0119.222212
TATGCGA203.8608996E-4108.75145
ACGCACT1750.0103.571434
GCACGCA1750.0103.571432
AGCACGC1750.0103.571431
GTAGTAA2700.0102.037041
AAACACG800.099.68755
AGTAACA2750.097.5454563
TAACAAG2750.094.9090965
CGCACTT1950.092.948725
GAAGCGG950.091.578941
CACGCAC2050.088.4146353
GCACTTG1100.085.6818166
CGGAGCC353.4296783E-582.857143
GTAACAA3300.079.0909044
ACAAGAG3500.076.642857
GAAACAC1050.075.952384
Produced by FastQC (version 0.11.5)