FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_D5_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_D5_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207229
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5170.24948245660597695No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT3760.18144178662252872No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC3380.16310458478301781No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA3330.16069179506729273No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT3120.15055807826124723No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT3020.14573249882979697No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2930.14138947734149176No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2930.14138947734149176No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2830.13656389791004156No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2590.12498250727456099No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT2520.12160460167254585No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT2500.12063948578625579No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC2400.11581390635480555No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC2380.11484879046851551No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG2350.11340111663908044No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC2340.11291855869593542No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA2340.11291855869593542No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG2180.10519763160561504No Hit
GATCTATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGC2170.10471507366247002No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2140.10326739983303496No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC2100.10133716806045485No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGAG100.007086835145.05
GAGCAGA151.2286095E-4145.01
CACAGGC100.007086835145.05
GTAACAA1400.0129.464284
CACGCAC700.0113.928573
GCACGCA700.0113.928572
AGCACGC700.0113.928571
CAGCCTT203.8616854E-4108.755
TCCCTAC203.8616854E-4108.753
GAAACAC650.0100.384614
ACGCACT850.093.8235244
CGCACTT850.093.8235245
AAACACG700.093.214295
TAGAAAC1000.087.02
TAGTAAC2250.080.555562
TAACAAG2400.075.5208365
ACAAGAG2400.075.5208367
CCTACAG300.001933569872.55
TGCGATA406.650737E-572.5145
GCGGAGC300.001933569872.51
Produced by FastQC (version 0.11.5)