FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_D4_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_D4_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208354
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC15030.7213684402507271No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG14070.6752930109333154No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG8960.4300373402958427No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA8120.3897213396431074No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA7690.36908338692801673No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA7250.34796548182420306No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA7020.33692657688357314No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA5700.27357286157213206No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT5350.2567745279668257No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA5350.2567745279668257No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC5050.24237595630513453No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA4940.23709648002918113No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA4940.23709648002918113No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC4890.23469671808556591No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA4800.23037714658705857No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG4440.21309886059302915No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC4000.19198095548921548No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT3850.1847816696583699No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT3740.17950219338241646No Hit
GGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCACCTGGGGA3650.17518262188390912No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT3630.17422271710646303No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG3530.16942319321923266No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT3370.16174395499966404No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA3360.161264002610941No Hit
GCACAGCTCTGGGAACTGGGAAAAGTGCTTCGGAAGGCTGGGCTGTCCGC3320.15934419305604883No Hit
CACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA3230.1550246215575415No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA3200.15358476439137236No Hit
CTCTCTGCCCAGTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCT3180.1526248596139263No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC3120.14974514528158805No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3100.148785240504142No Hit
AAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3090.14830528811541893No Hit
GTAGTAACAAGAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTA3090.14830528811541893No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3080.14782533572669593No Hit
GTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGACGTGAAAGCTGAAGA2990.14350576422818856No Hit
GAAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG2990.14350576422818856No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT2980.14302581183946553No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2940.14110600228457337No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC2930.14062604989585034No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT2880.13822628795223516No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT2820.1353465736198969No Hit
CATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG2780.13342676406500475No Hit
GTCCTACTGCAGCCTGTTGCCAAGGAGCTCCCCACAGACATGGAGCTCTC2720.13054704973266654No Hit
GATATAGCCACAGCCAAGGCATGCTGGCGGCCGCCAGAAGCTGGAAGAGG2700.12958714495522045No Hit
ATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCTTGCTT2670.12814728778905132No Hit
AGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA2550.12238785912437487No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA2550.12238785912437487No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2520.12094800195820575No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT2480.11902819240331358No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT2370.11374871612736016No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT2370.11374871612736016No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC2360.11326876373863712No Hit
TTTCAGATGTGATTAAGTATCTTGAGATGGGGAGAGTATTCTGGATTATT2360.11326876373863712No Hit
GTCCCATGGTGGGTGAAAATCTATCCTGGATAACTCCTGGACCAGTACCA2350.11278881134991409No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG2320.11134895418374498No Hit
GTCCTGGAGGCTGTAAATGCTAACTTATTTGGAAAAAGGGTCTTTTCAGA2310.11086900179502193No Hit
CACTAAAGGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCAC2290.10990909701757585No Hit
GTAGTAGGTTGAATAATTCCCTGCCCTCTCCCACTCAGTCCTCAAAGATA2250.10798928746268369No Hit
CCTTATAAGAGACAAGCAGAGGGAGATGTAACATGCAGGCACACAACAGG2250.10798928746268369No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG2220.10654943029651458No Hit
GTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCTTGGAAGCCAGA2220.10654943029651458No Hit
CCCTACAGCCACTTCTTCAGAAATTGAACTTGCTCTAAAATCCACTTCAT2200.1055895255190685No Hit
TCCCATGGCTGTAGAATCCATCAAGGCTTTGCCGATCTCTTGACCTGTTT2160.10366971596417636No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2150.10318976357545331No Hit
GTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGCTCACTCTCAC2130.10222985879800725No Hit
ACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACC2130.10222985879800725No Hit
AGCACAGCTCTGGGAACTGGGAAAAGTGCTTCGGAAGGCTGGGCTGTCCG2120.10174990640928419No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA2120.10174990640928419No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCGT100.007086863145.08
TATACTA100.007086863145.02
AGCACGC3450.0130.289861
TAGTAAC2700.0123.5185242
GCGGTGC650.0122.6923141
GTAGTAA2900.0117.51
GCACGCA3850.0116.753252
CGCACTT3850.0116.753255
ACGCACT3900.0115.256414
TAACAAG3000.0111.1666645
GCACTTG2500.0110.2000056
CACGCAC4100.0109.634153
AGTAACA3100.0107.580653
GCACTTC2600.0105.961546
GAAGCGG900.0104.722221
GTAACAA3200.0104.218754
CACTTGA2650.098.490577
GGCACTT1400.088.035715
AACAAGA3850.086.623386
ACTTCAG2800.085.446438
Produced by FastQC (version 0.11.5)