FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_D1_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_D1_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196778
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAATAGGGAGTTCAGTTTCAGATCTGTTCAATCTGTGATGTGTATTTAA2380.12094848001300959No Hit
GTATTTAACATGAAAACGGAGATGCCAAATAGGCACCGGCTAGACAAGTG2070.10519468639786968No Hit
GTCTATAGGAATGCCATATAATGGACACCTAAAATTATGAGCTATTATAA2060.10468649950705872No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT300.00193342472.51
TCGGCTA553.2126403E-452.7272769
GCGAAAG1004.6773494E-850.752
GAGCAAA3650.049.6575361
GTCTATA1109.904761E-846.1363681
AGCGAAA1109.904761E-846.1363681
CTGCGTG803.723657E-545.31259
AACAAGG4150.041.927712
ACTGCGT1053.8084345E-641.428578
CACTCGG700.001054571141.428576
ACTCGGC700.001054571141.428577
GCTAGAT1053.8084345E-641.428571
CAAAAGC4700.040.1063844
GCTTAGC750.001480488838.6666648
CGGGCCT750.001480488838.666664145
GTATACT951.0258828E-438.1578941
CGAAAGC1406.573737E-736.253
ACTGTCC1209.52486E-636.258
AAACAAG4850.035.876291
GCAAAAG5100.035.5392153
Produced by FastQC (version 0.11.5)