FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_D12_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_D12_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136099
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5100.3747272206261618No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA3590.2637785729505728No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC3090.2270406101440863No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA3040.22336681386343765No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA2980.21895825832665927No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT2950.21675398055827008No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC2570.18883312882534037No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC2240.1645860733730593No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT2240.1645860733730593No Hit
ATGTTATATTGATCTACTGTTGTTTGTTTCAGCACTGTTTTGGCTTCATT2210.16238179560467011No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC2090.15356468453111338No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2060.1513604067627242No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2060.1513604067627242No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2010.14768661048207554No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT1930.14180853643303773No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA1860.13666522164012962No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT1830.13446094387174043No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1820.1337261846156107No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA1800.13225666610335124No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA1710.12564383279818367No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1690.12417431428592422No Hit
ATTCAATACATCAAGAATTGAAAAGAACCCTTCATTAAGGATGAAGTGGG1690.12417431428592422No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT1620.11903099949301611No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC1610.11829624023688638No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT1590.11682672172462694No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTT1580.1160919624684972No Hit
GGACAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA1570.11535720321236746No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTTGATAAAG1560.11462244395623773No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT1480.1087443699071999No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT1460.10727485139494045No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT1430.10507057362655126No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG1420.10433581437042153No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT1410.1036010551142918No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA1390.10213153660203235No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG1380.10139677734590262No Hit
ATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT1380.10139677734590262No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT1380.10139677734590262No Hit
CAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT1380.10139677734590262No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAGCG151.2276889E-4145.05
CACGCAC450.0145.03
GCACGCA450.0145.02
AGCACGC450.0145.01
GAAACAC600.0108.754
AAACACG600.0108.755
TAACCTC203.8588027E-4108.755
CAGAAGC203.8588027E-4108.754
TATAGCA301.5944726E-596.6666642
ACAACGC406.0889215E-790.6258
CAACGCA406.0889215E-790.6259
ACCGGTC259.3692E-487.08
GCACCGG259.3692E-487.06
CCGGTCT259.3692E-487.09
ACGCACT850.085.294124
CGCACTT850.085.294125
AACACGA702.7885108E-972.500016
GAAGCGG300.001932137172.56
TAGCCCT300.001932137172.54
GTATAGC300.001932137172.51
Produced by FastQC (version 0.11.5)