FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_D10_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_D10_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173294
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT6290.3629669809687583No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA3820.22043463709072444No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3640.21004766466236569No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC3080.17773263932969405No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2950.17023093702032385No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG2920.1684997749489307No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA2870.1656145048299422No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2820.16272923471095366No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA2470.14253234387803387No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT2390.1379159116876522No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT2340.13503064156866365No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2330.13445358754486594No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2290.13214537144967511No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA2270.1309912634020797No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA2250.12983715535448428No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2100.12118134499751866No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC2100.12118134499751866No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT2040.11771902085473242No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT1990.11483375073574388No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA1990.11483375073574388No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT1990.11483375073574388No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG1980.11425669671194617No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA1970.11367964268814847No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1900.1096402645215645No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT1880.10848615647396909No Hit
CCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACT1860.10733204842637367No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT1850.10675499440257598No Hit
GATCTGGACGTAGCCTTGGGCAGACCAAAATGCACGGGGAAAATACCCTC1840.10617794037877826No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA1810.10444677830738514No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC1790.10329267025978972No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG1780.10271561623599201No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT1740.10040740014080117No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT151.2282644E-4145.06
CAGCATC100.0070858398145.04
CAGAGCG151.2282644E-4145.04
TAGTAGA100.0070858398145.03
GTTGGGT100.0070858398145.09
GTAACAA2000.0123.254
ACGTGCA550.0118.636372
CACGCAC256.449678E-6116.03
GCACGCA256.449678E-6116.02
AGCACGC256.449678E-6116.01
GAAACAC950.0114.473694
AGAGCGT203.8606054E-4108.755
AGCGTTT203.8606054E-4108.757
CGTTTTC203.8606054E-4108.759
CAACGCA352.75204E-7103.571439
AAACACG1100.098.863645
TAACAAG2900.085.05
ACACGTG353.4292447E-582.857147
TAGTAAC3050.080.819672
AACACGA1050.075.952386
Produced by FastQC (version 0.11.5)