FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_C7_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_C7_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184891
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC9280.5019173458956898No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG7050.3813057423022213No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA6650.35967137394464843No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA5040.2725930413054178No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA4810.2601532794998134No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG4280.2314877414260294No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA4080.220670557247243No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG4030.21796626120254634No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA3700.20011790730754875No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC3500.18930072312876237No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA3440.18605556787512642No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA3400.18389213103936916No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT3360.18172869420361185No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3210.17361580606952204No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3190.1725340876516434No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT3100.16766635477118952No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT3050.16496205872649292No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC3020.16333948109967494No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT3000.1622577626817963No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC2950.1595534666370997No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT2920.15793088901028174No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA2900.1568491705924031No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2890.15630831138346377No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT2860.15468573375664582No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG2860.15468573375664582No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT2820.1525222969208885No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA2810.15198143771194922No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC2800.15144057850300988No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2760.14927714166725262No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT2690.14549112720467736No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC2680.144950267995738No Hit
GGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCACCTGGGGA2650.14332769036892007No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA2630.1422459719510414No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2600.14062339432422347No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC2430.131428787772255No Hit
CTCTCTGCCCAGTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCT2430.131428787772255No Hit
GCACAGCTCTGGGAACTGGGAAAAGTGCTTCGGAAGGCTGGGCTGTCCGC2420.1308879285633157No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2420.1308879285633157No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG2380.1287244917275584No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA2350.12710191410074043No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA2340.1265610548918011No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT2260.12223418122028655No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC2260.12223418122028655No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA2250.12169332201134722No Hit
GTCCTGGAGGCTGTAAATGCTAACTTATTTGGAAAAAGGGTCTTTTCAGA2210.11952988517558993No Hit
ACCCAATGGTGTTCTCGCAACTCTACGGTATCCTGCAGCAGGAAACGAAT2190.1184481667577113No Hit
GTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGACGTGAAAGCTGAAGA2170.11736644833983266No Hit
GTCTATGATAAGCGTGAAAATCCCCTCCTCCCCGAGGAGGAGGAACAGAG2140.1157438707130147No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2140.1157438707130147No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA2130.11520301150407537No Hit
TCCTTCGACTGTCCTTGCAGCTGGGATAAGAGTCCCTGACTCTGGTTATG2040.1103352786236215No Hit
GGCATGATGTGTCCCCCATGGCCATTGAAGAGTTGATTTGAACGATGACG2030.10979441941468217No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA2010.10871270099680352No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA2000.10817184178786421No Hit
TTTCAGATGTGATTAAGTATCTTGAGATGGGGAGAGTATTCTGGATTATT1950.1054675457431676No Hit
ATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCTTGCTT1930.10438582732528895No Hit
GTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGCTCACTCTCAC1860.1005998128627137No Hit
GTCCCATGGTGGGTGAAAATCTATCCTGGATAACTCCTGGACCAGTACCA1850.10005895365377437No Hit
AGCAGGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC1850.10005895365377437No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCG100.0070862197145.09
ATAAGGC100.0070862197145.09
GGGTTAG151.2283967E-4145.01
AGCACGC2750.0126.5454561
GCACGCA2800.0124.285712
ACGCACT3000.0118.4166644
CGCACTT3000.0118.4166645
CACGCAC3050.0114.098363
AAACACG850.0102.352945
GCACTTG2050.099.024396
ACACGTG750.096.6666647
GCACTTC1800.096.6666646
AACACGT750.096.6666646
TAGTAAC2100.093.2142942
GTAGTAA2150.091.046521
TAGAAAC1200.090.6252
TAACAAG2200.088.977285
TCATTAC259.3745615E-487.03
GCGGTGC1050.082.857151
CACTTGA2300.081.956537
Produced by FastQC (version 0.11.5)