FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_C5_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_C5_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166692
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4310.2585606987737864No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3280.19677009094617617No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT3150.18897127636599237No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT3030.18177237059966883No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT3000.17997264415808797No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2620.15717610923139683No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2600.15597629160367624No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2570.15417656516209535No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT2300.13797902718786745No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT2290.13737911837400715No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2220.1331797566769851No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT2070.1241811244690807No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT1960.11758212751661747No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC1960.11758212751661747No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT1940.11638230988889689No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA1930.1157824010750366No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA1910.114582583447316No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC1870.11218294819187484No Hit
AGTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATT1830.10978331293643366No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC1800.10798358649485279No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA1770.1061838600532719No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG1750.10498404242555132No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA1740.10438413361169101No Hit
CTAGCAAAAGGATCCTTTGGAAAAGCACTAAGAGTAATATTTACTAAATG1730.10378422479783074No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG1720.10318431598397043No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG1700.10198449835624986No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT1690.10138458954238956No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTTTAAAATCAATTCC1690.10138458954238956No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAATTT1680.10078468072852927No Hit
GAGTAATATTTACTAAATGCTTGATGCACTATGTATTTGGAAATGCCCAA1680.10078468072852927No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG1670.10018477191466896No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCATC100.0070855995144.999986
GAAGCGG100.0070855995144.999981
GTAACAA1850.0133.243264
GAAACAC700.0124.285714
AAACACG750.0116.05
CACGCAC750.0116.03
GCACGCA750.0116.02
AGCACGC750.0116.01
CGCACTT850.0110.8823555
ATAAATG203.860345E-4108.749998
ACGTGCA203.860345E-4108.749992
ACGCACT900.0104.722224
CAGAGCA451.0861186E-896.6666644
TAACAAG2600.094.807695
TAGAAAC950.091.578952
AACACGA900.088.6111156
GCAGAGC750.087.03
AGCATCT502.257002E-887.07
TAGTAAC2900.085.02
GAGGAGT353.4288605E-582.857146
Produced by FastQC (version 0.11.5)