FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_C4_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_C4_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230045
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC16010.6959507922363016No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA15800.6868221434936643No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA12210.5307657197504836No Hit
GTAGTAACAAGAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTA10610.46121411028277076No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT9790.42556891043056794No Hit
CACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA8720.379056271599035No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG8470.36818883261970486No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA8290.36036427655458714No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT6910.3003760133886848No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG6760.29385555000108676No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA6650.2890738768501815No Hit
CATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG6360.27646764763415854No Hit
CACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA6020.2616879306222696No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA5840.25386337455715186No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5820.25299397943880547No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA5110.2221304527375079No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG5100.22169575517833467No Hit
ACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACC4960.2156099893499098No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA4630.20126496989719403No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT4540.1973526918646352No Hit
GTAGTAACAAGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT4530.19691799430546197No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA4470.19430980895042274No Hit
GTAGAAACACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT4290.18648525288530504No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG4280.18605055532613185No Hit
GCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCAT4160.18083418461605338No Hit
GTAGAAACACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT4130.17953009193853378No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3950.17170553587341608No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT3950.17170553587341608No Hit
TTCTAGGATAGTTAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGG3800.16518507248581799No Hit
CAAGAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA3620.1573605164207003No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3620.1573605164207003No Hit
GGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATT3550.15431763350648786No Hit
AAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCG3470.15084005303310222No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA3470.15084005303310222No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT3450.1499706579147558No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA3420.1486665652372362No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT3400.14779717011888976No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC3270.14214610184963813No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA3230.14040731161294528No Hit
GCACAGCTCTGGGAACTGGGAAAAGTGCTTCGGAAGGCTGGGCTGTCCGC3090.13432154578452044No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT3030.1317133604294812No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA3000.13040926775196157No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2910.12649698971940274No Hit
CAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTA2900.12606229216022952No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC2890.12562759460105632No Hit
GGATTAAGGCGACAGCGATTTCTAGGATAGTTAGTAGAATTAGAATTGTG2880.1251928970418831No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG2850.1238888043643635No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2790.12128061900932427No Hit
AGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACAT2780.12084592145015105No Hit
AGTAGAAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCT2760.11997652633180464No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC2730.11867243365428504No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT2700.1173683409767654No Hit
GTAGAAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTA2700.1173683409767654No Hit
AAGCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA2610.11345606294420657No Hit
ATCTAGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGAC2590.11258666782586015No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC2530.10997848247082093No Hit
AGTTAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGGGAAGGTTAA2500.10867438979330131No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT2480.1078049946749549No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA2480.1078049946749549No Hit
CATATCAGCAAGTCCTCCAAGTGGGTCCCTATCCATTTTCATCCTGGGTG2480.1078049946749549No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC2460.1069355995566085No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA2460.1069355995566085No Hit
CTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCT2450.1065009019974353No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT2450.1065009019974353No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA2420.10519680931991567No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG2410.10476211176074246No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT2370.10302332152404964No Hit
TTTCAGATGTGATTAAGTATCTTGAGATGGGGAGAGTATTCTGGATTATT2350.10215392640570323No Hit
AGCAGGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC2320.10084983372818362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC4200.0127.738091
GCACGCA4300.0124.767452
TAGTAAC3950.0121.1392442
GTAGTAA4350.0111.6666641
CACGCAC4850.0110.618553
ACGCACT4900.0109.489794
AGTAACA4400.0108.749993
CGCACTT4950.0108.383855
GTAACAA4450.0107.528094
GCACTTC1950.0104.102576
TAACAAG4600.0104.021735
CACTTGA3000.0101.500017
GCACTTG3100.0100.564526
GCGGTGC800.099.68751
CGGTGCA607.2759576E-1296.6666642
ACAACGC608.258212E-1084.5833368
GAAGCGG1050.082.857141
ACAAGAG5750.081.956527
CGAGCAA451.2298588E-680.555565
ACTTCAG2300.078.8043448
Produced by FastQC (version 0.11.5)