FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_C12_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_C12_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140313
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT7750.5523365618296238No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA4980.3549207842466485No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC4760.3392415528140657No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA3610.257281933961928No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA3030.21594577836693676No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2990.21309500901555808No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT2940.2095315473263347No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC2720.19385231589375182No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG2640.1881507771909944No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT2310.16463193004212012No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2290.16320654536643078No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC2290.16320654536643078No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC2220.15821769900151803No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC2180.15536692965013932No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT2180.15536692965013932No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG2170.15465423731229466No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2130.15180346796091596No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGGACAATT2110.15037808328522662No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC2060.14681462159600323No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT1980.1411130828932458No Hit
ATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT1940.13826231354186713No Hit
CTGATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCC1870.13327346717695437No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1860.13256077483910972No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT1760.12543385146066294No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA1720.12258308210928424No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC1700.12115769743359489No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA1700.12115769743359489No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC1700.12115769743359489No Hit
AGACAATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC1650.11759423574437151No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA1570.1118926970416141No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT1530.1090419276902354No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC1520.10832923535239072No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG1490.1061911583388567No Hit
GTGATGAATCTTCTTTTTCTTTCCTGGTCTTTGGGTTTTAAAAAGCAGAT1480.10547846600101202No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG1480.10547846600101202No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG1480.10547846600101202No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACTTAGACTCTGCCTT1480.10547846600101202No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA1460.10405308132532268No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA1460.10405308132532268No Hit
GTAATAAAAGGGTCCTTGCCTTTAATTGGTGAAGCAGATTGCCTTCATGA1450.103340388987478No Hit
CTGTATATCAGTTAAGCATCTTTTGTTTTTTATCCATTCCAAGGCAGAGT1450.103340388987478No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA1440.10262769664963332No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA1440.10262769664963332No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT1430.10191500431178864No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTGAT1420.10120231197394397No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT1410.10048961963609929No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGAA100.007084413145.04
CAGGAGT100.007084413145.04
TACTACG100.007084413145.05
AACACGT151.2277703E-4145.06
GCACGCA1200.0126.8752
AGCACGC1200.0126.8751
CACGCAC1250.0121.7999953
GAAACAC550.0118.636364
ACGCACT1300.0117.115394
CGCACTT1300.0117.115395
TGTTATA256.445369E-6116.02
CGTGCCT203.8590576E-4108.754
ACGTGCC203.8590576E-4108.753
CAAGAGG650.0100.384628
TAACAAG2100.0100.119055
GTAACAA2200.095.568184
AAACACG700.093.214295
TAGAAAC850.085.294122
ACACGTG353.426966E-582.857141
TATAGAG353.426966E-582.857145
Produced by FastQC (version 0.11.5)