FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_C11_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_C11_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182794
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC7410.4053743558322483No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA6610.36160924319178966No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA6060.33152072825147433No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA5510.301432213311159No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA4540.2483670142346029No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG4520.2472728864185914No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG4430.24234931124653983No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT4320.23633160825847674No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA4030.22046675492631052No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT3970.2171843714782761No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA3770.2062430933181614No Hit
CTATAATTGTGAGTCGGGGTCTTAACCTGACTAATTTATCAGCTAGAAGA3740.2046019015941442No Hit
GTGTATGTAGTGGTTTAGAGCTGGAATTTGGCATAACTGGGTTTTTATTT3610.19749007079006967No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG3350.1832664091819206No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3260.17834283400986903No Hit
GTCTTAACCTGACTAATTTATCAGCTAGAAGAGATAAAGCCAAGGGATGC3260.17834283400986903No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA3190.1745133866538289No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA3110.17013687538978303No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT3070.1679486197577601No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT2940.16083678895368558No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG2860.15646027768963972No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA2830.1548190859656225No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA2790.1526308303335996No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA2750.15044257470157663No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC2690.14716019125354224No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT2680.1466131273455365No Hit
GATAATGGCCTCTGTCAAAGCAAAGAATAGTTGTAGAGACACAAACTGGG2590.14168955217348492No Hit
CTCTTCTTCTTTTCCAAAAGTGTTTTGATGCACTTATGAAGGATAGATTC2570.14059542435747344No Hit
CTCTAGTTGTGTCCTAATACATCTGTTCTGGATTCCCATGCTACTGGTAA2540.13895423263345624No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC2520.13786010481744476No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2500.1367659770014333No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA2400.131295337921376No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT2370.12965414619735877No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG2330.12746589056533583No Hit
GCATTAAAGGTATCCATTGATGATTCTGTCCATTCATTTAACACTACACA2330.12746589056533583No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC2200.12035405976126132No Hit
GTGCTACAGATTAGCATTTACCATTGTTTTAGGTGCTTTACATGTAACAA2170.11871286803724411No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC2110.11543048458920971No Hit
GTCCTCATTATCTGACAGAGGCACTCCAAATCCTCTCCCATATCTTTGAG2060.11269516504918105No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC2050.11214810114117532No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA2040.11160103723316958No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2040.11160103723316958No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2040.11160103723316958No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC1990.10886571769314092No Hit
TCCTTATACAAGAGTAAACTTATTCTTATCAACCTGATGGTGGGTCAAAG1970.10777158987712945No Hit
AATCTATCCTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGA1940.10613039815311225No Hit
ATCTATAATTGTGAGTCGGGGTCTTAACCTGACTAATTTATCAGCTAGAA1940.10613039815311225No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT1940.10613039815311225No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA1930.1055833342451065No Hit
GTCCAGGAGTTATCCAGGATAGATTTTCACCCACCATGGGACGTCATCGT1930.1055833342451065No Hit
CAGTAGACCCATCCAAGACATCCAGATGCTAGATCTGTCTAAAATGTCAG1920.10503627033710076No Hit
GATTAGCATTTACCATTGTTTTAGGTGCTTTACATGTAACAACCTATTTA1900.10394214252108933No Hit
GGCATGATGTGTCCCCCATGGCCATTGAAGAGTTGATTTGAACGATGACG1850.10120682298106064No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAG100.0070861555145.06
TTGTACG100.0070861555145.03
TGCGCGA100.0070861555145.05
TTCGCGC100.0070861555145.03
GCACGCA1900.0137.368422
AGCACGC1900.0137.368421
CACGCAC2000.0130.53
GAAGCGG800.0117.81251
CGCACTT2400.0108.755
ACGCACT2450.0106.530614
GCGGTGC1000.0101.51
GCACTTC1650.0101.060616
TAGTAAC2950.090.93222
TGGACGA259.3743904E-487.0145
CGGTGCG259.3743904E-487.02
GTAGTAA3100.086.532261
TAACAAG3150.085.158735
ACAACGC608.240022E-1084.5833368
CAACGCA608.240022E-1084.5833369
AAGCGGA950.083.947372
Produced by FastQC (version 0.11.5)