FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_C10_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_C10_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249646
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT11010.4410244906788012No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA6700.26838002611698164No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC6440.2579652788348301No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA5980.2395391874894851No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG5590.22391706656625784No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT5280.21149948326830795No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5110.20468984081459346No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA5070.20308757200195476No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT4640.18586318226608878No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC4140.16583482210810507No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT3510.1405990883090456No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA3470.1389968194964069No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT3340.13378944585533115No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT3330.13338887865217147No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT3320.1329883114490118No Hit
GTGTAACTGTTATCTCTACAGGCACATTCTATGGTTTTATTGCTGGCAAA3260.13058490823005375No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC3190.12778093780793603No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG3190.12778093780793603No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT3070.12297413137001995No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT3050.12217299696370061No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT3040.12177242976054092No Hit
CCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACT3040.12177242976054092No Hit
CAAGTAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTT3020.12097129535422159No Hit
GGAAAATACAGGGCTTAACTTCCCTTATAGACAGATGGAGCAAGAAACAT2980.11936902654158286No Hit
GTATCAGTCTCCACGTTTAATTTGACAAAAGGTCTTTTTGCTGTGTAACT2970.1189684593384232No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2960.11856789213526353No Hit
GACACATACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGC2960.11856789213526353No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAGCA2940.11776675772894418No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA2880.11536335450998614No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2870.11496278730682646No Hit
GTGTTTTACTCTTCCTGTTGGAAATATTTCTTTTATTATTCGGCCCTCTC2720.10895427925943135No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG2670.106951443243633No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG2630.10534917443099429No Hit
TATGATAACTGATGGCTCAGCTTCAGGTGTTAGTGAATGCAGATTTCTTA2630.10534917443099429No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA2620.10494860722783461No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG2620.10494860722783461No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT2600.10414747282151526No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT2560.10254520400887657No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAATTT2550.1021446368057169No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT2540.10174406960255722No Hit
ATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACATAC2530.10134350239939754No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA2510.1005423679930782No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC2500.10014180078991852No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACT100.007087697145.04
AAACACG500.0145.05
CACGCAC500.0145.03
CGGACTC100.007087697145.05
GCACGCA500.0145.02
AGCACGC500.0145.01
GTAACAA2700.0131.574074
CAGCATC256.4552987E-6116.05
GAAACAC650.0111.538464
GAGCAGA203.8626214E-4108.751
ACGTGCA800.0108.752
TAGTAAC3450.0102.9710162
CAACGCA301.5970973E-596.6666649
AGTAACA3700.096.013523
CACGTGC1150.094.565221
AACACGA554.1472958E-1092.272726
ACGCACT800.090.6254
CGCACTT800.090.6255
CAGAAGC259.378439E-487.04
TAGAAAC950.083.9473652
Produced by FastQC (version 0.11.5)