FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_B9_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_B9_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203574
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT7800.3831530549087801No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA5410.2657510291098077No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA5350.26280369791820174No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC5120.2515055950170454No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT5100.2505231512865101No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT4880.23971627025062137No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT4560.22399717056205606No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT4490.2205586175051824No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4240.20827807087349073No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA4140.20336585222081408No Hit
GAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAG4090.20090974289447575No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT4080.20041852102920804No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC3750.18420819947537503No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA3750.18420819947537503No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC3680.18076964641850138No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT3630.17831353709216305No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC3560.17487498403528937No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC3560.17487498403528937No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC3510.17241887470895104No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA3410.16750665605627438No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC3140.15424366569404738No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGATGAAGAA3110.15277000009824437No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA2930.14392800652342638No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC2910.14294556279289103No Hit
GTCCAAAGGTCCTTAGCTCAAGAGCATGGAATAGAGACTCCTAAGTATCT2880.14147189719708805No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT2840.13950700973601737No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT2780.13655967854441137No Hit
CTCCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAGAGATTTATA2620.1287001287001287No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT2540.12477035377798737No Hit
CCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAGAGATTTATAGA2500.1228054663169167No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT2480.12182302258638136No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC2460.12084057885584604No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT2460.12084057885584604No Hit
GATTTATAGAAGTTGGAATAACAAAAGGATTGGCCGATGATTACTTTTGG2440.11985813512531068No Hit
TTCTAATGGTATGCTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTC2420.11887569139477537No Hit
GTATAGCACCGGTCTTATTCTCCAATAAAATAGCCAGACTGGGAAAAGGA2410.11838446952950771No Hit
TTCTAATGCTGTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCAC2410.11838446952950771No Hit
ATACAAGGATATGACATGACTAAAGCTTGTTTCAAGGGAGACAGAGTAAA2360.11592836020316936No Hit
GGTTTATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCA2320.1139634727420987No Hit
TTATTATTGTCATTCCTATTGCCATGTCTGCTGATTCATTTACTCCAGCA2310.11347225087683104No Hit
GGACAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA2300.11298102901156336No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA2260.1110161415504927No Hit
CTCTATTGGTTATTGGTATTACTTTGTATTTTCCCATGCTGGCATTGCTT2240.11003369781995735No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC2230.10954247595468969No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC2190.10757758849361902No Hit
TCTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAGATTTCTTTCT2180.10708636662835136No Hit
GTATGGAAGGGATAAACGACTTTTACCGAACATGCAAATTATTGGGAATA2110.10364781357147769No Hit
CCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATTTT2080.1021741479756747No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA2060.10119170424513935No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT2060.10119170424513935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAC306.475602E-10145.04
AGAAGCG100.007086744145.05
AGCGGTG100.007086744145.08
AAGCGGT100.007086744145.07
CGCGGTT100.007086744145.04
TCCGCGG100.007086744145.02
CTCCGCG100.007086744145.01
AAGCACG100.007086744145.01
CCGCGGT100.007086744145.03
AGCACGC600.0120.8333361
CACGCAC650.0111.538473
GCACGCA650.0111.538472
TGACTAT203.8615867E-4108.751
AAACACG404.796675E-9108.755
GACTATC203.8615867E-4108.752
ACTATCC203.8615867E-4108.753
AACACGT203.8615867E-4108.756
ACAACGC700.0103.571438
AACAACG750.096.6666647
ATATAGG451.08721E-896.6666643
Produced by FastQC (version 0.11.5)