FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_B8_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_B8_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207191
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT4680.22587853719514844No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT4450.21477766891419028No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC4090.19740239682225577No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3800.18340564985930854No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT3550.17133948868435406No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT3010.14527658054645232No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2860.1380368838414796No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2760.1332104193714978No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2630.12693601556052145No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTTTAAAATCAATTCC2620.12645336911352328No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2550.123074843984536No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT2550.123074843984536No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT2520.12162690464354146No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT2520.12162690464354146No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2490.12017896530254692No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT2490.12017896530254692No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG2480.11969631885554875No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG2470.11921367240855056No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAATTT2460.11873102596155237No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT2310.11149132925657969No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA2260.10907809702158879No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT2240.1081128041275924No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA2210.10666486478659787No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA2200.1061822183395997No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAA2170.10473427899860516No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2150.1037689861046088No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC2130.10280369321061242No Hit
AGTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATT2130.10280369321061242No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA2130.10280369321061242No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC2120.10232104676361425No Hit
AAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTATTTCAT2120.10232104676361425No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA2120.10232104676361425No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCTGAGTCCACTCTTGGATC2110.10183840031661606No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA2080.10039046097562153No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA100.007086833145.01
TAGTAGA100.007086833145.03
ATTGTGA151.228609E-4145.08
AGCACGC500.0145.01
CATAAGG151.228609E-4145.01
CACGCAC600.0132.916673
GCACGCA550.0131.818192
GTAACAA1650.0131.818184
CGCACTT650.0122.6923145
ACGCACT700.0113.928574
CAGCATC203.8616837E-4108.755
CTAGCAT203.8616837E-4108.753
CGTGCCT203.8616837E-4108.753
ACGTGCC203.8616837E-4108.752
GAAACAC700.0103.571434
CGGTGCG301.5964528E-596.6666642
GGTGCGT301.5964528E-596.6666643
TAGAAAC800.090.6252
GAGCGTT259.3761674E-487.07
AGCGTTT259.3761674E-487.08
Produced by FastQC (version 0.11.5)