FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_B6_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_B6_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73936
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT2660.3597706124215538No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA1850.2502164033758926No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA1290.17447522181346029No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA1260.17041765851547286No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1210.1636550530188271No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1190.1609500108201688No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT1170.1582449686215105No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC1140.15418740532352304No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC1120.15148236312486477No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT1120.15148236312486477No Hit
ATTGTATTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACC1090.14742479982687728No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC1080.14607227872754816No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA1080.14607227872754816No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT1050.1420147154295607No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTGAATTAATGGAT1040.14066219433023155No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT1040.14066219433023155No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC1010.1366046310322441No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT940.12713698333694004No Hit
GCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGTGAGACGAGAAAT880.11902185674096516No Hit
GAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAG860.11631681454230686No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCCGGAGT860.11631681454230686No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT830.11225925124431942No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC830.11225925124431942No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC820.11090673014499026No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC790.10684916684700282No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT790.10684916684700282No Hit
CACGTGCATTTTTCACTCAATTGTATTCATTGAATTAATGGATAAATTTA790.10684916684700282No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC790.10684916684700282No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT780.10549664574767367No Hit
GGACAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA770.10414412464834451No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA770.10414412464834451No Hit
GTATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG760.10279160354901536No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA740.10008656135035707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAC500.0145.04
AAACACG500.0145.05
AACACGT151.2254364E-4145.06
CACGCAC100.0070776753144.999983
GCACGCA100.0070776753144.999982
CGTGCTC100.0070776753144.99998145
AGCACGC100.0070776753144.999981
ATATAGG100.0070776753144.999983
ACACGAG458.367351E-11112.777787
AACACGA458.367351E-11112.777786
ACACGTG203.8517502E-4108.749997
TAGAAAC750.096.6666642
GTCATAA406.0612365E-790.624991
GAAGCGG259.352138E-487.01
CCTGAGA259.352138E-487.03
AGCGGAG259.352138E-487.03
CACGAGC608.1490725E-1084.5833368
TAGTAAC851.8189894E-1276.76472
GTAGTAA950.076.315791
ACGCACT300.001928631272.54
Produced by FastQC (version 0.11.5)