FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_B5_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_B5_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89447
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2480.2772591590550829No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1920.2146522521716771No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT1730.19341062305052154No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA1650.184466779210035No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1570.17552293536954844No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1380.15428130624839292No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC1320.147573423368028No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT1270.1419835209677239No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG1230.13751159904748061No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA1230.13751159904748061No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA1210.13527563808735898No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC1200.13415765760729817No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC1170.13080371616711572No Hit
CCCCCAGACCAAACGATGGAAGCATAACAGGCCCTTGTGAATCTAATGGG1160.12968573568705488No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA1150.1285677552069941No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT1130.12633179424687246No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT1070.11962391136650753No Hit
CCATTGGATTGTGCAAACGCATCAAATGTTCAGGCTGTGAACCGTTCTGC1040.11626996992632509No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT1030.11515198944626427No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC1030.11515198944626427No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1010.11291602848614263No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTTTAAAATCAATTCC960.10732612608583855No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT940.1050901651257169No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA930.10397218464565608No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA930.10397218464565608No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG930.10397218464565608No Hit
CTAGCAAAAGGATCCTTTGGAAAAGCACTAAGAGTAATATTTACTAAATG920.10285420416559526No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC910.10173622368553445No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT900.10061824320547363No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT900.10061824320547363No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA900.10061824320547363No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTT100.007080144144.999984
CAGAGCG100.007080144144.999982
AGCGTTT100.007080144144.999985
CAACGCA100.007080144144.999989
GTAACAA1250.0121.7999954
CACGCAC203.8544263E-4108.749993
GCACGCA203.8544263E-4108.749992
AGCACGC203.8544263E-4108.749991
CTCGCTG203.8544263E-4108.749991
GAAACAC352.7410533E-7103.571434
AAACACG406.071732E-790.624995
GCTGTCA259.358611E-487.04
TAACAAG1750.087.05
ACGTGCA259.358611E-487.02
CGCTGTC259.358611E-487.03
TAGTAAC2000.076.1252
ACAAGAG2100.075.9523857
AGTAACA2100.072.500013
TAGAAAC502.28908E-672.52
ACACGAG300.001929961672.57
Produced by FastQC (version 0.11.5)