FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_B4_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_B4_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45986
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA6631.4417431392162832No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA5841.2699517244378724No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA3770.8198147262210237No Hit
CACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA3680.8002435523855087No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3610.7850215282912191No Hit
CACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTA2880.626277562736485No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2760.6001826642891315No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2700.5871352150654546No Hit
GTAGAAACACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT1960.4262166746401079No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT1910.4153438002870439No Hit
GTAGAAACACGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT1840.40012177619275435No Hit
AAGCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA1740.37837602748662635No Hit
GTAGTAACAAGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCT1740.37837602748662635No Hit
ACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT1660.360979428521724No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC1550.33705910494498326No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC1480.32183708085069374No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG1360.29574218240334016No Hit
GGATTAAGGCGACAGCGATTTCTAGGATAGTTAGTAGAATTAGAATTGTG1330.2892184577915018No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA1300.28269473317966337No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA1280.2783455834384378No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC1200.26094898447353543No Hit
TTTCAGATGTGATTAAGTATCTTGAGATGGGGAGAGTATTCTGGATTATT1140.24790153524985864No Hit
GGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATT1020.22180663680250512No Hit
GAAGCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATG1010.21963206193189233No Hit
GAAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATG990.21528291219066673No Hit
GAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCTCT980.21310833732005394No Hit
TTCTAGGATAGTTAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGG920.20006088809637718No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT910.19788631322576436No Hit
AAACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCG910.19788631322576436No Hit
GTCCTGGAGGCTGTAAATGCTAACTTATTTGGAAAAAGGGTCTTTTCAGA890.19353716348453878No Hit
AGCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAA880.191362588613926No Hit
GGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCACCTGGGGA830.180489714260862No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA820.1783151393902492No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG810.17614056451963642No Hit
AATGAGGACAGTGGGACCTAGATTAGACCATGAACGAGCCAAGTCCTTAA800.17396598964902363No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA800.17396598964902363No Hit
CATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG780.16961683990779802No Hit
GAAGCAGAGCGATGGTGAGAGCTAAGGTCGGGGCGGTGATGTAGAGGGTG760.16526769016657245No Hit
AGCACAGCCTTCAGATGCCAGAAGGGCAGAGGGAATCCCACACGGGCTGG760.16526769016657245No Hit
CACTAAAGGACTAGAGAGTGCTTTTACAGAAAAAGTGAGTACTTTCCCAC740.16091854042534687No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA730.15874396555473405No Hit
ACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACC720.15656939068412126No Hit
CGTGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAG710.15439481581350845No Hit
ACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAA700.15222024094289568No Hit
CCTTATAAGAGACAAGCAGAGGGAGATGTAACATGCAGGCACACAACAGG700.15222024094289568No Hit
GATTAAGGCGACAGCGATTTCTAGGATAGTTAGTAGAATTAGAATTGTGA680.14787109120167008No Hit
AACACGAGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGAT680.14787109120167008No Hit
GGCTTGGATTAAGGCGACAGCGATTTCTAGGATAGTTAGTAGAATTAGAA660.1435219414604445No Hit
GTAGTAACAAGAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTA660.1435219414604445No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG650.14134736658983169No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG650.14134736658983169No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC630.13699821684860608No Hit
ATTCAGGTGAGTTCTATGTGCAATTATGAATGTCCTTATAAGAGACAAGC630.13699821684860608No Hit
AGTTAGTAGAATTAGAATTGTGAAGATGATAAGTGTAGAGGGAAGGTTAA620.13482364197799332No Hit
AGATTATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC610.1326490671073805No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA610.1326490671073805No Hit
AGCAGGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGC600.13047449223676771No Hit
GAATAGGGGAAATCAGTGAATGAAGCCTCCTATGATGGCAAATACAGCTC580.12612534249554214No Hit
GTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCCAGCAA580.12612534249554214No Hit
CTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTAGG570.12395076762492932No Hit
GCACAGCTCATTGCCGGCTACATCGATATCATCCTGAAGAAGAAAAAAAG570.12395076762492932No Hit
CTCCCACTCAGTCCTCAAAGATAGCAGGCCCTAGTCCTGGAGGCTGTAAA570.12395076762492932No Hit
AAGCAGAGCGATGGTGAGAGCTAAGGTCGGGGCGGTGATGTAGAGGGTGA540.11742704301309094No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT530.11525246814247814No Hit
ATTCTGGATTATTCAGGTGAGTTCTATGTGCAATTATGAATGTCCTTATA520.11307789327186535No Hit
AGCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAA520.11307789327186535No Hit
GTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTAGGATAGTTAG510.11090331840125256No Hit
GATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGGACCTAGATTAGA500.10872874353063976No Hit
ATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGAA500.10872874353063976No Hit
AATCTATCCTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGA500.10872874353063976No Hit
AACAACAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGC500.10872874353063976No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT490.10655416866002697No Hit
GTAGTAGGTTGAATAATTCCCTGCCCTCTCCCACTCAGTCCTCAAAGATA480.10437959378941417No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC480.10437959378941417No Hit
GATATGGGAGAGGATTTGGAGTGCCTCTGTCAGATAATGAGGACAGTGGG480.10437959378941417No Hit
GTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAGGAATGCCATT480.10437959378941417No Hit
AATTAGAATTGTGAAGATGATAAGTGTAGAGGGAAGGTTAATGGTTGATA480.10437959378941417No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA470.10220501891880138No Hit
GAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTG470.10220501891880138No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG470.10220501891880138No Hit
GTATTCTGGATTATTCAGGTGAGTTCTATGTGCAATTATGAATGTCCTTA460.10003044404818859No Hit
AAGCGGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA460.10003044404818859No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCG100.0070690196145.02
GGATTAG151.2224412E-4145.01
GATTAGG151.2224412E-4145.02
GCGGTGC256.3989446E-6116.01
TAGTAAC501.8189894E-12116.02
GAAGCGG600.0108.751
GTAGTAA553.6379788E-12105.454551
ACGCACT501.891749E-10101.54
GCACGCA451.0684744E-896.6666642
CGGAGCG301.5831945E-596.6666645
AGCACGC451.0684744E-896.6666641
CACGCAC554.0381565E-1092.2727363
CGCACTT554.0381565E-1092.2727365
GAGCGAT406.024511E-790.6257
AGCGATG406.024511E-790.6258
GCGATGG406.024511E-790.6259
TAACAAG651.4551915E-1189.2307745
GAAACAC1000.087.04
AAACACG1000.087.05
GCACTTG502.2206223E-887.06
Produced by FastQC (version 0.11.5)