FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_B1_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_B1_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161544
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA3340.20675481602535534No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA3330.20613578963006984No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3020.18694597137621946No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2850.17642252265636607No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2630.16280394196008519No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC2610.16156588916951417No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2510.15537562521665924No Hit
TTCTTATACAGTTCCCCCTAAAACAGAAATTGAATGTACTTACTTGAGCA2230.1380428861486654No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC1790.1108057247561036No Hit
CATATGTACTAAGAATTTTCAAAATTGGTTTGTTCAGTGTGGAGTATATT1670.10337740801267767No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC1620.10028227603625019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG502.2566383E-887.09
AGCGAAA1051.0986696E-955.2380941
ATTCGAC400.00604173854.3750041
ATATAGA400.00604173854.3750041
TCGACAC400.00604173854.3750043
GAGCGAA752.5410114E-548.3333361
GAGCAAA4850.047.8350521
TAGAAAC2600.047.4038432
TCTACAC803.7206715E-545.3125043
CAAAAGC4800.045.31254
GTAGAAA3050.045.1639371
CGAAAGC1307.2850526E-944.6153833
AGCAGGC1505.311449E-1043.58
GGTCGTT350.003658129641.428577
AGGTCGT350.003658129641.428576
TAAACTA1953.6379788E-1240.8974385
AAGCAGG6300.040.2777757
GACAGTA1157.1056165E-637.8260847
AGTAGAA2500.037.71
AGCAAAA6000.037.4583362
Produced by FastQC (version 0.11.5)