FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_B12_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_B12_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158700
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT4190.2640201638311279No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3930.24763705103969755No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC3290.20730938878386895No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2740.17265280403276623No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG2730.1720226843100189No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA2610.16446124763705106No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT2500.1575299306868305No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA2370.14933837429111532No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2360.148708254568368No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT2270.1430371770636421No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2240.14114681789540012No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2230.1405166981726528No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC2230.1405166981726528No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT2210.13925645872715817No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA2200.13862633900441082No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG2180.1373660995589162No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2180.1373660995589162No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC2160.13610586011342157No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA2120.13358538122243227No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT2090.13169502205419029No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT2040.1285444234404537No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG2030.12791430371770637No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2000.1260239445494644No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT2000.1260239445494644No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT2000.1260239445494644No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG1960.1235034656584751No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1920.12098298676748581No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG1900.11972274732199117No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT1880.11846250787649654No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA1850.11657214870825457No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT1840.11594202898550725No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC1800.11342155009451795No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT1790.11279143037177064No Hit
CTCCTAATTAGCCCTCATAGATTCGGAGAAACCAAAGGAAACTCAGCTCC1740.10964083175803402No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAGGAGG1740.10964083175803402No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT1700.10712035286704474No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA1690.10649023314429741No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA1690.10649023314429741No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTGCTAGTTTTCCTTCT1690.10649023314429741No Hit
ACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAACAAGGGGAAA1680.10586011342155008No Hit
GTATATGCTTTTCCTTCTTCGTCAAGAAAATTCATGTCACTTATTACATA1670.10522999369880277No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC1670.10522999369880277No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA1650.10396975425330812No Hit
ATCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAA1630.10270951480781348No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC1620.10207939508506615No Hit
TTTGTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTT1610.10144927536231885No Hit
CAATAGACACCGTGATCAGAACGCATGAGTACTCAAACAAGGGGAAACAG1600.10081915563957151No Hit
GACACATACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGC1600.10081915563957151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCAC202.125029E-6144.999983
GCACGCA202.125029E-6144.999982
CTTATGT100.0070852833144.999985
CCTTATG100.0070852833144.999984
AGCACGC202.125029E-6144.999981
CAACGCA100.0070852833144.999989
GTAACAA1150.0138.695664
ACAACAG301.0973963E-7120.8333361
CAGCCTT203.8600015E-4108.749995
ACGCACT301.5952957E-596.6666644
ATAGTAC301.5952957E-596.6666648
TAACAAG1650.096.6666645
GCAGAAG259.372099E-487.01
CGCACTT259.372099E-487.05
GAAACAC353.428355E-582.857144
AAACACG353.428355E-582.857145
TAGTACC353.428355E-582.857149
TAGAAAC451.2280943E-680.555552
TAGTAAC2000.079.752
AGAGCAC607.988092E-872.56
Produced by FastQC (version 0.11.5)