FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_B11_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_B11_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115472
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4130.35766246362754606No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC2970.2572052099210198No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA2930.2537411666897603No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT2870.248545101842871No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT2680.23209089649438827No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2640.22862685326312873No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA2560.22169876680060968No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT2420.20957461549120132No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA2360.20437855064431204No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2320.2009145074130525No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT2230.19312041014271858No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2210.1913883885270888No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA2200.19052237771927394No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA2000.17320216156297633No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT1950.16887210752390192No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC1920.16627407510045725No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC1830.15847997783012333No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT1780.15414992379104892No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC1780.15414992379104892No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC1730.1498198697519745No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT1700.14722183732852986No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC1700.14722183732852986No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT1690.146355826520715No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA1680.1454898157129001No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT1590.13769571844256617No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT1590.13769571844256617No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC1590.13769571844256617No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1550.13423167521130663No Hit
GGACAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA1530.13249965359567686No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT1500.12990162117223222No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG1500.12990162117223222No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC1480.12816959955660245No Hit
GATTAAGAATGGATGCACGACTAGATTATGAATCAGGAAGAATGTCAAAG1470.12730358874878758No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC1420.12297353470971317No Hit
TTCTAATGCTGTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCAC1410.1221075239018983No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC1400.12124151309408343No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA1380.11950949147845366No Hit
ATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT1370.11864348067063878No Hit
GGTTTATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCA1370.11864348067063878No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC1340.11604544824719414No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG1340.11604544824719414No Hit
ATGTTATATTGATCTACTGTTGTTTGTTTCAGCACTGTTTTGGCTTCATT1320.11431342663156437No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTTGATAAAG1320.11431342663156437No Hit
ATACAAGGATATGACATGACTAAAGCTTGTTTCAAGGGAGACAGAGTAAA1310.11344741582374948No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTT1300.1125814050159346No Hit
CTCAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTT1280.11084938340030484No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG1280.11084938340030484No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA1260.10911736178467507No Hit
CCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATTTT1250.10825135097686019No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC1240.1073853401690453No Hit
GATTTATAGAAGTTGGAATAACAAAAGGATTGGCCGATGATTACTTTTGG1240.1073853401690453No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGATGAAGAA1230.10651932936123043No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT1230.10651932936123043No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG1210.10478730774560066No Hit
GATATAACCCCAGCACATGGTCCCGTGAAGAAAATGGATTATGATGCAGT1200.10392129693778578No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT1190.10305528612997089No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCAAACAAGGGAAAACAA1170.10132326451434114No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC1160.10045725370652625No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG1160.10045725370652625No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC1160.10045725370652625No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGAG100.0070827976145.02
CGGTGCC100.0070827976145.02
GGTAAGA100.0070827976145.01
CGATTCC100.0070827976145.0145
CACCCAC256.4404903E-6116.08
ACGCACT458.367351E-11112.777784
CACGCAC458.367351E-11112.777783
GCACGCA458.367351E-11112.777782
AGCACGC458.367351E-11112.777781
CGCACTT458.367351E-11112.777785
TTTAAGA203.8573053E-4108.759
GTCATAA451.0830263E-896.6666641
ACCCACA301.5934444E-596.6666649
AACACGA259.3655783E-487.01
GTAGTAA1550.084.193551
ACACGAG300.001931393372.52
GCCTTTA300.001931393372.56
TGTTATA300.001931393372.52
TAGTAAC1800.072.52
CATACAA300.001931393372.53
Produced by FastQC (version 0.11.5)