FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_B10_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_B10_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206088
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA16990.8244051084973409No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT7600.3687745040953379No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA6480.31442878770234073No Hit
GATAATGGCCTCTGTCAAAGCAAAGAATAGTTGTAGAGACACAAACTGGG5980.29016730716975275No Hit
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC5570.27027289313303055No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA5490.26639105624781645No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT5330.2586273824773883No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA5120.24843756065370134No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG5110.24795233104304956No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT5080.2464966422110943No Hit
AAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCAAAGCAAAGAA4740.22999883544893443No Hit
GTCCTGGAGGCTGTAAATGCTAACTTATTTGGAAAAAGGGTCTTTTCAGA4280.20767827335895347No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA4170.2023407476417841No Hit
GCCATATACCGATGGGGTTATCCATGCAACTATCTCCATTCCTACAATGT3800.18438725204766895No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA3750.18196110399441015No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3630.17613834866658903No Hit
GGATAAAATATCCCAGGAATTAATTAGAGTTTTTTTCGTCCAATGTCTCT3520.17080082294941967No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA3400.16497806762159853No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG3380.16400760840029502No Hit
TTTCAGATGTGATTAAGTATCTTGAGATGGGGAGAGTATTCTGGATTATT3310.16061100112573268No Hit
GTGTATGTAGTGGTTTAGAGCTGGAATTTGGCATAACTGGGTTTTTATTT3300.16012577151508092No Hit
CTATAATTGTGAGTCGGGGTCTTAACCTGACTAATTTATCAGCTAGAAGA3230.1567291642405186No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3210.1557587050192151No Hit
CTCTTCTTCTTTTCCAAAAGTGTTTTGATGCACTTATGAAGGATAGATTC3180.1543030161872598No Hit
CTTATAAGGACATTCATAATTGCACATAGAACTCACCTGAATAATCCAGA3130.151876868134001No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA3110.1509064089126975No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA3010.1460541128061799No Hit
GAATAGTTGTAGAGACACAAACTGGGCCCTTGAAGCTGTGACTAACATTT2920.14168704631031404No Hit
GCTTTATGGTATGTGTCATGAATACAAATGACAGCCAATTTGGCACCTTA2900.14071658708901053No Hit
ATATATGCCAATAAGAAAACATTCTTTGCAAAAGTCTTCCATGCAAATAA2870.13926089825705523No Hit
GTCCTCATTATCTGACAGAGGCACTCCAAATCCTCTCCCATATCTTTGAG2850.13829043903575172No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA2720.13198245409727882No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT2710.13149722448662707No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA2630.127615387601413No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT2610.12664492838010946No Hit
GTCTTAACCTGACTAATTTATCAGCTAGAAGAGATAAAGCCAAGGGATGC2580.1251892395481542No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA2580.1251892395481542No Hit
TTATCTGACAGAGGCACTCCAAATCCTCTCCCATATCTTTGAGTTGGACT2560.12421878032685067No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2540.12324832110554715No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT2470.11985171383098482No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG2440.11839602499902954No Hit
GTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATA2400.11645510655642251No Hit
GCTAAGATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAA2370.11499941772446721No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT2350.11402895850316369No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT2330.11305849928186018No Hit
AATCTATCCTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGA2290.11111758083925313No Hit
GAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGAAACATCAAATT2270.11014712161794961No Hit
GCATTAAAGGTATCCATTGATGATTCTGTCCATTCATTTAACACTACACA2270.11014712161794961No Hit
ACTTTGAGAAGGAGATCAACAATCACACTCAAGAAGAAAAGAAGTTATTT2240.10869143278599434No Hit
CTCTAGTTGTGTCCTAATACATCTGTTCTGGATTCCCATGCTACTGGTAA2220.10772097356469082No Hit
TACAAGTACCACTCAACATTTCCCTGTTTACAAAAAAAGCTATAAGCAGA2200.1067505143433873No Hit
GGCTATATCACTCCAGTCTTCAAGAGCTGCATCTTCAAGTCTCTCTCTAT2200.1067505143433873No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG2130.10335390706882497No Hit
GTAGTAGGTTGAATAATTCCCTGCCCTCTCCCACTCAGTCCTCAAAGATA2100.10189821823686969No Hit
CAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGAAGAAAAG2080.10092775901556617No Hit
AGTAGAAACAACGCACTTTGGGATAGTAACTGATGTTTGTGTTTGGTGAG2070.1004425294049144No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATC100.0070868065145.04
CGACCGA100.0070868065145.01
AGCACGC2550.0119.411761
GCACGCA2650.0114.9056552
CACGCAC2800.0108.753
GTAGTAA4050.0103.827161
TGCTTAG650.0100.384611
GCACTTC1250.092.7999956
TAGTAAC4550.090.824182
ACAGCGG406.1001447E-790.6258
AGTAACA4600.089.836963
GCACTTG1700.089.558836
AAACACG1850.086.216225
TAGAGTA1150.081.956535
TAACAAG5050.081.831685
AAGCGGA1250.081.21
GCTTAGA1000.079.752
ACGCACT4150.078.6144564
CACTTGA1700.076.764717
CGCACTT4300.075.87215
Produced by FastQC (version 0.11.5)