FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_A9_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_A9_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204770
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC6780.33110318894369295No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA6240.3047321384968501No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA6150.30033696342237637No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT5890.2876397909850076No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5470.2671289739707965No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT4760.23245592616105873No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT4700.22952580944474288No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC4380.21389852029105827No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT4360.21292181471895297No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT4290.20950334521658448No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA4090.19973628949553154No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA3690.18020217805342578No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC3610.17629535576500463No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC3490.1704351223323729No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT3430.16750500561605702No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC3370.16457488889974117No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGATGAAGAA3260.15920300825316208No Hit
TTCTAATGCTGTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCAC3190.15578453875079357No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2960.14455242467158275No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA2910.14211066074131953No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT2720.1328319578063193No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT2580.12599501880158226No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC2570.12550666601552962No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT2530.12355325487131905No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC2510.12257654929921376No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC2440.11915807979684524No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT2420.11818137422473995No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA2370.11573961029447673No Hit
GGACAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA2350.11476290472237144No Hit
ATTTAGTACTATGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTA2300.11232114079210821No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAACCT2290.11183278800605558No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT2280.11134443522000292No Hit
ATTCAATACATCAAGAATTGAAAAGAACCCTTCATTAAGGATGAAGTGGG2240.10939102407579236No Hit
CCAATAGAGTGGTAAATGAAAAGGGAGAAAGTTTCGACATGCTTTATGGT2230.1089026712897397No Hit
GAATAGAGACTCCTAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAG2220.10841431850368707No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2190.10694926014552913No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT2180.10646090735947648No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG2180.10646090735947648No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTT2170.10597255457342385No Hit
ATACAAGGATATGACATGACTAAAGCTTGTTTCAAGGGAGACAGAGTAAA2160.1054842017873712No Hit
GATTTATAGAAGTTGGAATAACAAAAGGATTGGCCGATGATTACTTTTGG2160.1054842017873712No Hit
ATCCAATGATGACCAATAACCCCATAGACATCTTCGGAGCTTATGTGTAC2140.1045074962152659No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTTGATAAAG2110.10304243785710797No Hit
GATTAAGAATGGATGCACGACTAGATTATGAATCAGGAAGAATGTCAAAG2100.10255408507105533No Hit
GTAATAAGTGACATGAATTTTCTTGATGAAGAAGGAAAAGCATATACAGC2070.1010890267128974No Hit
ACTTCATATTGTGGTCTCAAATTTTGTTCTTTCCCTTGTCCTTCTAATGC2050.10011232114079212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACC100.0070867743145.04
CACGTAC100.0070867743145.03
TCACGTA100.0070867743145.02
AAACACG850.0127.941185
AGCACGC700.0124.285711
GAAACAC850.0119.4117664
CACGCAC750.0116.000013
GCACGCA750.0116.000012
CTTGATT256.452503E-6116.09
CGCACTT850.0110.8823555
TAGAAAC950.0106.8420942
ACACGAG750.0106.3333367
AACACGA750.0106.3333366
ACGCACT900.0104.722224
GGAGCGT352.7538408E-7103.571433
CGGAGCG352.7538408E-7103.571432
ACGAGCC451.08721E-896.6666649
ACACGTG301.5964084E-596.666667
AACACGT301.5964084E-596.666666
GCGGAGC651.4551915E-1189.2307741
Produced by FastQC (version 0.11.5)