FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_A8_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_A8_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235582
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT7600.32260529242471836No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4940.20969344007606694No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT4910.20841999813228518No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC4800.20375071100508527No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT4160.17658394953774056No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA3590.1523885526058867No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT3550.15069063001417765No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA3360.1426254977035597No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT3340.14177653640770518No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT3040.12904211696988735No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC2890.12267490725097843No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA2880.12225042660305117No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT2840.12055250401134213No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA2840.12055250401134213No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAATTT2790.1184301007717058No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG2770.11758113947585129No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT2740.11630769753206952No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2710.11503425558828773No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2700.11460977494036047No Hit
AGTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATT2700.11460977494036047No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2700.11460977494036047No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2660.11291185234865142No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT2660.11291185234865142No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2610.11078944910901513No Hit
GTATACACTCTGTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTC2580.10951600716523334No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG2510.10654464262974252No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT2480.10527120068596071No Hit
GTATATGCTTTTCCTTCTTCGTCAAGAAAATTCATGTCACTTATTACATA2430.10314879744632442No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG2400.10187535550254263No Hit
TCCAAATACATAGTGCATCAAGCATTTAGTAAATATTACTCTTAGTGCTT2400.10187535550254263No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC2370.10060191355876086No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC2370.10060191355876086No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACACT100.0070874454145.04
CCTAGGT151.2288213E-4145.04
TAGGTAG151.2288213E-4145.06
TCCTAGG151.2288213E-4145.03
GCTGTAC100.0070874454145.01
TGTACAC100.0070874454145.03
TACACTT100.0070874454145.05
GCACGCA500.0130.52
GTAACAA1900.0125.921064
CACGCAC550.0118.636373
AGCACGC550.0118.636371
CAACGCA256.4545384E-6116.09
AGGTAGT203.862348E-4108.757
GAAGCGG404.798494E-9108.751
GAAACAC651.6370905E-1189.2307744
ACGCACT750.087.04
ACGTGCA259.3777775E-487.02
CGCACTT750.087.05
TAGTAAC2850.086.491232
TAACAAG2900.085.000015
Produced by FastQC (version 0.11.5)