FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_A7_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_A7_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261792
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGATTTTTATTCATTAGGAACGACTGAGCAGGCCTCAGGC13320.5088008800880087No Hit
AGCACGCACTTCAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTG9270.3540979097909791No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA8500.324685246302408No Hit
AAGCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA7910.30214827038259384No Hit
GAAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATG6260.2391211343356558No Hit
GGTAGAGGCTTACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACA6110.23339139469502504No Hit
GCACAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT5890.22498777655543334No Hit
CTCCTGGACCAGTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCA5590.21352829727417183No Hit
AGATTAGACCATGAACGAGCCAAGTCCTTAATGGATCAGTACTTTGCCCG5030.1921372692824838No Hit
ATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCACTGTCCTCAT4680.17876787678767878No Hit
GTCTAATCTAGGTCCCACTGTCCTCATTATCTGACAGAGGCACTCCAAAT4310.16463451900745632No Hit
AAGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA4140.15814081408140815No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA4060.15508495293973842No Hit
GTCCTTAATGGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTA4020.15355702236890356No Hit
AAGCAGAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGA4010.15317503972619484No Hit
GCAGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3970.15164710915536No Hit
AGATCATTTTACCTGTCCAGGGGCCTGAGCCAGCAGCAGAAATGCCAGAC3900.14897323065639897No Hit
CAGTGATACTGTCCATCTCCTCCTCCTCCACGTCGCCTGGGTCAGCAGGC3780.1443894389438944No Hit
CTCCAAATCCTCTCCCATATCTTTGAGTTGGACTCTCTTCTTCTTTTCCA3770.14400745630118567No Hit
GGCTAATCTCATCTGCATTAAGCTGTCCTTTCTTAGAAAACCGAGGTGGC3740.14286150837305953No Hit
CCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGATCGTCTTTGGT3730.1424795257303508No Hit
AAATAATCTTCTCTGGCTCCTCCCTTCCAGCAAAACATCCTGCAAGGCTC3690.14095159515951594No Hit
GCGGAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3670.14018762987409852No Hit
GTAGTAACAAGAGCAATGACAGCTGATGTTTGTCGATGGTTTCCTCTGTA3520.13445789023346777No Hit
GATCAAGATGTTGGGAAACATCAAATTCATTGGAGAGCTTGGCAAGCTTG3500.13369392494805035No Hit
GGAATAAACACCCCTAGATCTTTTACTGCATCTTGACGAATTTGATTGAT3500.13369392494805035No Hit
GTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGG3450.1317840117345068No Hit
CTTCATAAGTGCATCAAAACACTTTTGGAAAAGAAGAAGAGAGTCCAACT3340.12758220266471093No Hit
GCTCTATTGTACACCAGTTTTCTCGGTTTATCGCCTGGGTCATTGGGCCT3290.12567228945116735No Hit
GCGGTGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAAT3260.12452634152304118No Hit
GTACTGATCCATTAAGGACTTGGCTCGTTCATGGTCTAATCTAGGTCCCA3190.12185246302408019No Hit
GTACCAATTCCGCTACCTGGCATTTGTCCAAACATATCAGCAAGTCCTCC3140.1199425498105366No Hit
AGCACAGCTGTACCATGGGTGACGCTGAGGAAGCCCAAATAGACGATGAA3080.11765065395428433No Hit
CTCTAGTCCTTTAGTGAGAGGACCCAACTCAGGGCTGGACAGTGACATGT3070.11726867131157559No Hit
GATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCC3060.11688668866886688No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG2930.1119209143136536No Hit
CATTCAGACGCCTCCTAATTTCCAAATTACAAGATGAATTTGAAAACCGA2870.10962901845740129No Hit
CAATAGAGCCATGCCCTGATGCAGCATCTCTTCTGGCTTCCAAGCAGAGC2810.107337122601149No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA2740.10466324410218801No Hit
CTTATGAAGGATAGATTCGTGAATAAGATCAAGCTTGCCAAGCTCTCCAA2710.10351729617406186No Hit
CTCTCTGCCCAGTCCAACATCCAGGAAGTTCTCACATTCTGGGCTCTGCT2670.101989365603227No Hit
CCATTGGGTTCCTCGCAAGGCTTTTCTTGACAATGGACCAAAGACGATCA2630.10046143503239213No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC100.007087893145.06
TATGCGA100.007087893145.0145
GAGCGAT151.2289763E-4145.07
ACGTGGT100.007087893145.04
AGCACGC3900.0122.692311
GCACGCA4100.0116.707312
GCACTTG3050.0109.344276
GGCGGAA203.8628338E-4108.758
CACGCAC4400.0108.749993
CGCACTT4400.0108.749995
ACGCACT4450.0107.528084
AAACACG1300.0105.961545
CACTTGA3150.096.6666647
GAAGCGG1650.092.272731
AAGCACG554.1472958E-1092.272722
ACGAGCT406.104783E-790.6259
GCACTTC2650.087.547176
CGGCGGA259.378952E-487.07
CCCCGTT259.378952E-487.09
GCGGCGG259.378952E-487.06
Produced by FastQC (version 0.11.5)