FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_A6_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_A6_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261520
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC21270.8133221168553074No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG20480.783114102171918No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA17880.6836953196696237No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC17160.6561639645151423No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA16920.6469868461303151No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT14150.5410676047721015No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA13420.5131538696849189No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG12280.46956255735698993No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC9590.36670235546038543No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT8160.3120220250841236No Hit
GCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGG7980.30513918629550324No Hit
ATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA7890.30169776690119304No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTC7680.29366778831446927No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA6990.26728357295809113No Hit
TAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGG6950.2657540532272866No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG6570.25122361578464364No Hit
TAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAA6500.24854695625573572No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG6050.23133985928418477No Hit
CAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCG5680.21719180177424288No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA5540.21183848271642702No Hit
ATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGAT5520.21107372285102477No Hit
GCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTC5420.20724992352401345No Hit
AGCGAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACG4920.18813092688895686No Hit
CCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGAACC4890.18698378709085348No Hit
CACTTGGAGGTGTGTTTCATGTATTCAGATTTCCATTTCATCAATGAACA4890.18698378709085348No Hit
GTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGTTTGTCCCTAAGAG4630.17704190884062404No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGGACAGGGTACA4600.17589476904252063No Hit
GTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATA4570.17474762924441725No Hit
GCATTAAGCATCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAA4540.17360048944631384No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA4440.16977669011930255No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT4400.168247170388498No Hit
GCTTAATGCTGGTCCGTATCTTCTGTCTTCTTTACCTATAATGAGGAACC4380.16748241052309576No Hit
ATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGCCC4310.16480575099418782No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCC4210.1609819516671765No Hit
CTCCTCAATTGCTTCGTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA4200.16059957173447537No Hit
CCTCAATTGCTTCGTATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGAGT3900.14912817375344142No Hit
GTCAGTAAGTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGT3860.1475986540226369No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG3830.14645151422453348No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3830.14645151422453348No Hit
ATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCAGTC3770.14415723462832672No Hit
CATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGATGCTTAAT3490.133450596512695No Hit
ATGCTAAGTCAGTATTCAATAGCTTGTATGCATCACCACAATTGGAAGGA3470.13268583664729275No Hit
AAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAAT3460.13230345671459162No Hit
AGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACG3410.13039155705108596No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3380.12924441725298258No Hit
ACAAATAGTAGCACTGCTATAACTATTTTAATGCATGTGTCAGGAAGGAG3220.12312633832976445No Hit
TCAATGAACTGAGTAACCTTGCAAAAGGGGAAAAGGCTAATGTGCTAATT3200.1223615784643622No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCGTATAGCCCCCCAAGATCAAA3170.12121443866625879No Hit
AGGTTACTCAGTTCATTGATGCTTAATGCTGGTCCGTATCTTCTGTCTTC3100.11853777913735086No Hit
GGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATG3040.11624349954114407No Hit
GCTTCAACCCGATGATTGTCGAACTTGCAGAAAAAGCAATGAAAGAGTAT3030.11586111960844295No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC2940.11241970021413276No Hit
AAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTC2890.1105078005506271No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCGTATAGCCCCCC2860.10936066075252371No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA2840.10859590088712144No Hit
GGACCATATGGCCATAATTAAGAAGTACACATCGGGGAGACAGGAAAAGA2830.10821352095442031No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT2790.1066840012236158No Hit
GTATTCAATAGCTTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA2780.10630162129091464No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT2770.10591924135821351No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC2760.10553686142551238No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT2750.10515448149281126No Hit
GTCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCA2750.10515448149281126No Hit
AGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTT2720.10400734169470788No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2700.1032425818293056No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC2640.10094830223309881No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCCC353.4325218E-582.857146
AGCGAAA1800.072.51
GCAGGGT903.6379788E-1272.59
GCTCCCC406.653997E-572.499997
AAGCAGG11600.068.750017
TAGAAAC2900.067.500012
GGTAGTT651.3940189E-766.923087
CAAAAGC11600.065.000014
GCATTGG451.1922163E-464.444441
TCGCTCC451.1922163E-464.444445
CGAAAGC2050.063.6585353
AGCAGGT9850.060.355338
GCAGGTC4700.060.1595769
GTAGAAA3300.059.318181
TAGTTTT753.7503924E-758.09
GCTTTCG502.0074876E-458.01
GCGAAAG2300.056.7391322
TTTTTGG3950.055.0632931
GAGCGAA953.1273885E-853.4210551
CAGGGTG553.2146001E-452.7272768
Produced by FastQC (version 0.11.5)