FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_A3_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_A3_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205583
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG6710.3263888551096151No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5680.2762874362179752No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG5240.2548848883419349No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT4700.22861812503952175No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG4590.22326748807051164No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG4260.20721557716348143No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4130.20089209710919675No Hit
ATCTATAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT4030.19602788168282398No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3730.18143523540370554No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3720.1809488138610683No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3670.17851670614788187No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC3670.17851670614788187No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA3630.17657101997733277No Hit
ATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATCGG3500.1702475399230481No Hit
CTCCAATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3340.16246479524085164No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC3020.14689930587645866No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTTCAAACAG3010.14641288433382138No Hit
GTACGTGATAGGACCCCTTATCGGACTCTATTGATGAATGAGTTGGGTGT2920.14203509045008583No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG2870.13960298273689944No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2780.13522518885316392No Hit
GAGCAAAAGCAGGTCAATTATATTCAGCATGGAAAGAATAAAAGAACTAC2740.1332795026826148No Hit
ATCACGTACTGTGTTATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC2710.13182023805470297No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT2700.1313338165120657No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA2690.1308473949694284No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA2630.1279288657136047No Hit
GGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCGGGA2630.1279288657136047No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT2600.12646960108569288No Hit
TCCCTGTACACATTGTGGTCATAAGTTTCATTTCTTATTGATTCTATGCA2570.12501033645778103No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG2550.12403749337250647No Hit
TACTATGATTCTTCCTGATGATTGAGCATACGGGAAGATTTGGTCCTTGT2540.1235510718298692No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2480.12063254257404551No Hit
CTATTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGTCTGGT2460.11965969948877096No Hit
GGATACCCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGT2440.1186868564034964No Hit
GAATAAGGGGTATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA2400.11674117023294728No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC2360.11479548406239815No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG2310.11236337634921176No Hit
CTTATAGATAGTGTTGTTTCATGGTCCAAAGATATTCTCAGGACCCAGGA2290.11139053326393719No Hit
GTACACATTGTGGTCATAAGTTTCATTTCTTATTGATTCTATGCAGGCAT2290.11139053326393719No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAAGAAATGGGACCT2280.11090411172129991No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2270.11041769017866264No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG2190.1065263178375644No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC2170.10555347475228984No Hit
GTATTTTAGTATCAGCTTTTCCTGTAGCATTTCCATCAGTCATTACTACT2160.10506705320965255No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG2150.10458063166701526No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTT2140.10409421012437799No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT2120.10312136703910343No Hit
CTATAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCATCC2120.10312136703910343No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTTCGTTCAACAAAAAGGTC2110.10263494549646614No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA2070.10068925932591702No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCCT100.0070867934145.04
TCGAGTG100.0070867934145.03
TAAGCCA100.0070867934145.05
GCCGTTA100.0070867934145.0145
GTCTACT259.376067E-487.09
TGTCTAC259.376067E-487.08
GCAGGGG750.087.09
AGGGTGT608.258212E-1084.5833366
GAGCGAA702.7976057E-972.51
GCTAAAT406.65061E-572.52
TAGAAAC1100.065.909092
GGGTATT350.003562474962.1428577
ACAAGGT702.3253415E-762.1428578
AAGCAGG5450.058.5321127
TATATAT502.0064724E-458.04
GCAGGGT651.0905736E-555.769239
GGTCCGC1051.1023076E-955.2380948
AGCGAAA1206.002665E-1154.3751
CGAAAGC1258.913048E-1152.23
GCAAAAG5300.050.6132053
Produced by FastQC (version 0.11.5)