FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_A12_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_A12_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216416
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT9190.4246451279018187No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA5390.249057370989206No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT4960.22918823007541034No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA4760.21994676918527278No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAG4630.2139398196066834No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC4460.20608457785006654No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA4190.1936086056483809No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA3820.1765119030016265No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT3510.16218763862191335No Hit
ATCATGTACCATCTCTATCCCAATACAGGGGACATCGCATTTCTTATCTT3440.15895312731036523No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT3440.15895312731036523No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG3310.15294617773177582No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT3300.15248410468726897No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA3290.1520220316427621No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT3070.14185642466361081No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT2980.13769776726304894No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAA2950.1363115481295283No Hit
TTTTACTAGTTTTCCTTCTTGAGTTCCAATACTGAAAGTTTTGGGGCTAT2860.13215289072896644No Hit
CAATAATGCATTGCTCAAAGTAAAATATGGAGAAGCATATACTGACACAT2820.13030459855093893No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA2810.12984252550643205No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT2750.1270700872393908No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG2680.12383557592784268No Hit
GAACAAAATAGTACCAAATTGAGTACCATTTCCTTTTATTCATCCATTAT2670.12337350288333579No Hit
CTACAATAAAGGAAAATACAGGGCTTAACTTCCCTTATAGACAGATGGAG2670.12337350288333579No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT2650.12244935679432206No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2610.12060106461629454No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT2590.11967691852728078No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC2580.11921484548277392No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2560.11829069939376016No Hit
CAAGTAAACAAGCACTACAATAAAGGAAAATACAGGGCTTAACTTCCCTT2560.11829069939376016No Hit
GGAAAATACAGGGCTTAACTTCCCTTATAGACAGATGGAGCAAGAAACAT2560.11829069939376016No Hit
CCTTAAGAGCACTCAAGAGGCCATAAACAAGATAACAAAAAATCTCAACT2550.11782862634925328No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2540.11736655330474642No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG2510.11598033417122579No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT2490.11505618808221203No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGTAAACAAGCA2490.11505618808221203No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG2480.11459411503770517No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTGTCCATTG2440.11274582285967766No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA2400.11089753068165016No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC2380.10997338459263639No Hit
TGTCATATCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGT2360.10904923850362266No Hit
GTGTAACTGTTATCTCTACAGGCACATTCTATGGTTTTATTGCTGGCAAA2330.10766301937010203No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT2320.10720094632559515No Hit
GAACCAAGCTGAGGCATCTAATTTCAGTCAAATTGGGCAAAATCCCAACA2300.10627680023658138No Hit
ATCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAA2280.10535265414756766No Hit
GTATTACCCAAGGGTTGTTGCTAATACATTCTTCTATTCCAGAATACATT2230.10304228892503327No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC2210.10211814283601951No Hit
GTCTAGGAGACTTCTACTGTTGATTCAAGCATTAAAGGACAGAAAGGGCC2190.10119399674700577No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA2180.10073192370249888No Hit
TCTCTGGACCATCCATGCTATGGTTCTTGGCATTCCCTCAATTACTTCAT2170.10026985065799202No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2170.10026985065799202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA400.0145.02
GAGCAGA100.0070870505145.01
CAGAGCG100.0070870505145.05
AGCACGC400.0145.01
AAACACG600.0120.8333365
GAGCATC900.0112.777786
GTAACAA2300.0110.326094
CGCACTT600.0108.755
GAAACAC900.096.6666644
CAGAGCA1300.094.8076864
AGCATCT1150.088.260877
TAACAAG2900.087.55
CCCTATG259.3767385E-487.04
TAGAAAC950.083.947372
GCAGAGC1550.079.516133
CACGCAC755.4569682E-1177.333333
ACGCACT851.8189894E-1276.76474
AACACGA851.8189894E-1276.76471
GAAGCGG406.651404E-572.51
ACGTGCA502.3001285E-672.52
Produced by FastQC (version 0.11.5)