FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_A11_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_A11_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239665
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT9590.4001418646861244No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA8220.3429787411595352No Hit
GTAAGAAGTACAGTAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA6890.2874846139402917No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC6800.2837293722487639No Hit
ATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT6390.2666221600984708No Hit
CTCAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC5520.23032149041370245No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT5480.2286524941063568No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC4840.20194855318882604No Hit
CAATTACTATGTGGTCACTCAATACCTCCATGTTGTCAGAGAGTACTTCC4740.1977760624204619No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA4690.1956898170362798No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA4420.18442409196169654No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT4330.1806688502701688No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT4150.17315836688711325No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT3990.16648238165773058No Hit
CAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATACGA3970.16564788350405776No Hit
GTCATAATGGATACCTTTATTACAAGAAACTTCCAGACTACAATAATACA3720.15521665658314732No Hit
GTATATGCTTTTCCTTCTTCATCAAGAAAATTCATGTCACTTATTACATA3630.15146141489161957No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG3580.14937516950743746No Hit
GGACAATAGGACATTTGAATCAAATAAAAAGAGGAGTAAACATGAAAATA3580.14937516950743746No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT3500.14603717689274612No Hit
TACCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATT3480.14520267873907328No Hit
GTGTGAATGTGATGCTTGTTTCTCGCACAAAGCACAGAGCGTTCCTAGTT3360.14019568981703628No Hit
ATATTACTCGTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT3340.13936119166336344No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC3320.13852669350969063No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC3280.13685769720234495No Hit
CCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATTTT3200.1335197045876536No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT3120.13018171197296224No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT3100.12934721381928943No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT2900.12100223228256107No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCCAAGTCCACTCTTGGGTC2830.11808148874470614No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT2810.11724699059103333No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAATTGTGT2780.11599524336052405No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA2780.11599524336052405No Hit
CAATACCTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT2720.11349174889950557No Hit
ATACAGTAACGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT2640.1101537562848142No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2620.10931925813114139No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA2610.10890200905430496No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA2600.10848475997746855No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGCTTAAAGTCAATTCC2600.10848475997746855No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG2580.10765026182379571No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC2580.10765026182379571No Hit
ATTCAATACATCAAGAATTGAAAAGAACCCTTCATTAAGGATGAAGTGGG2570.1072330127469593No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGCTTTCCTTGATAAAG2570.1072330127469593No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC2520.1051467673627772No Hit
TCCAAATACATAGTGCATCAAACACTTAGTAAATATTACTCGTAGTGCTT2510.10472951828594079No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT2500.10431226920910439No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG2450.1022260238249223No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC2400.10013977844074021No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCCAC100.007087522145.08
ACCCACA100.007087522145.09
CTCACTG100.007087522145.02
ATATAGG202.127268E-6145.03
CGGAGCG151.2288478E-4144.999982
GGAGCGT151.2288478E-4144.999983
AGCACGC1050.0138.095231
CACGCAC1100.0131.818183
GCACGCA1100.0131.818182
AACACGT301.09912435E-7120.833326
AAACACG501.8189894E-12116.05
GAAACAC553.6379788E-12105.4545444
ACGCACT1450.0105.04
CGCACTT1450.0105.05
ACGTGCA301.5969668E-596.666669
CAGCCTT301.5969668E-596.666665
TAGAAAC700.093.214292
ACACGTG406.1032006E-790.6257
GTAGTAA2650.087.547171
AACGTAT259.3779794E-487.06
Produced by FastQC (version 0.11.5)