FastQCFastQC Report
Fri 22 May 2020
000000000-J3T7H_l01_n01_A10_AR.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-J3T7H_l01_n01_A10_AR.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311353
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT6080.1952767437602978No Hit
CAACCAAGACTACTCGTTAAGTAATGAATCCTCATTGGATGAGGAAGGGA6010.19302849177621542No Hit
GGAATTTCCATTGCAAAATTAGATACAAATCCATCTCTATAGAACATGCT5910.1898167032275263No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT5700.18307194727527917No Hit
CATTTCAACTTCTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAG5700.18307194727527917No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT5670.18210841071067244No Hit
CATGTTAACTTTTTAGGTGTGACTGATACTAAGGGAGACATCCTTGCTAG5480.17600601246816314No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT5350.1718306873548673No Hit
ACTCATTTCCTCATTACTTATTGTATTTACACAGTCTCTCCAAAGCTTCC5140.16508593140262018No Hit
ATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA5130.16476475254775128No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC4920.15801999659550414No Hit
GACTCAGGAAAGTGGCCAAAATATACTGTGTTTAGGATTGGCTCCCTATT4660.14966934636891246No Hit
TCTCTGGACCATCCATGCTATGGTTCTTGGCATTCCCTCAATTACTTCAT4420.1419610538520586No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT4340.1393916230131073No Hit
GTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTGATTGA4270.13714337102902494No Hit
GTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTCCAGAATACATT4080.1310409727865156No Hit
CAAAAGGATTGGCCGATGATTACTTTTGGAAAAAGAAAGAAAAGCTGGGA4010.12879272080243326No Hit
GTTATTCATAGCTGATTATAGATACACCTACAAATGCCACAGGGGAGATT3980.12782918423782652No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAACTT3940.12654446881835088No Hit
ATATAAACCCAAAGGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC3930.12622328996348195No Hit
GGGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACC3880.1246173956891374No Hit
CCATAGACTCAACTGTCATTCTTTGTCTCTTAATTCCTTGTGAAATGTCA3800.12204796485018611No Hit
GGGTCTATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACC3660.11755146088202137No Hit
GGAATTTCCATTGCAAAATTAGATACAAATCCATCTCTGTAGAACATGCT3650.11723028202715247No Hit
GGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCA3600.1156243877528079No Hit
ATTGCGTTCATTGAATTAATGGATAAATTTATATTAGCTCAAGGCCCACC3570.11466085118820117No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAAC3430.11016434722003642No Hit
GTCCAAAGGTCCTTAGCTCAAGAGCATGGAATAGAGACTCCTAAGTATCT3420.10984316836516751No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGT3360.10791609523595404No Hit
GGTTTATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCA3290.10566784325187167No Hit
AGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTAAATTAT3190.10245605470318256No Hit
CATCAATACAAGGATATGACATGACCAAAGCCTGTTTCAAGGGAGACAGA3160.10149251813857582No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCG151.2291984E-4145.03
AGCACGC950.0122.105271
GCACGCA1000.0116.02
CACGCAC1050.0110.476193
GTCATCG203.8635297E-4108.752
GGAGCGT203.8635297E-4108.754
AGCGGAG352.7572423E-7103.571431
GCGTTCA501.9463187E-10101.54
ACGTGCA501.9463187E-10101.52
ACGCACT1200.096.6666644
CGCACTT1200.096.6666645
GCGGAGC406.1075116E-790.6252
GAAACAC651.6370905E-1189.2307744
AAACACG651.6370905E-1189.2307745
GTTCGCA259.380635E-487.03
AACACGT451.2308847E-680.555566
TAGAAAC851.8189894E-1276.76472
ACACGTG502.302506E-672.57
CGTTCAT702.8048817E-972.55
TGTTCGC300.001934486372.52
Produced by FastQC (version 0.11.5)